Osteopontin 抗体
Quick Overview for Osteopontin 抗体 (ABIN271757)
抗原
See all Osteopontin (SPP1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Osteopontin/SPP1 protein. (region surrounding Leu285)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-Secreted phosphoprotein 1 (SPP1) (AA 17-317) antibody
SPP1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 18-314) antibodySPP1 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 1-300) antibodySPP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F2 unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 167-314) antibodySPP1 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 7C5H12 unconjugated
anti-Secreted phosphoprotein 1 (SPP1) antibodySPP1 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal 7C5H12 unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 167-314) antibodySPP1 适用: 人 WB, ELISA, IHC, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 221-280) antibodySPP1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (C-Term) antibodyVerified SPP1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 1-300) antibodySPP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (C-Term) antibodySPP1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 山羊, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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别名
- Osteopontin / SPP1
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背景
- Osteopontin (OPN, also designated Bone Sialoprotein 1, Urinary Stone Protein, spp-1, eta-1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights between 44 and 75 kDa, due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.Synonyms: BNSP, Bone Sialoprotein 1, Nephropontin, OPN, SPP-1, Secreted Phosphoprotein 1, Urinary Stone Protein, Uropontin
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分子量
- approx. 35, 60 kDa
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基因ID
- 6696
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NCBI登录号
- NP_000573
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UniProt
- P10451
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途径
- Regulation of Cell Size
抗原
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