TLR4 抗体
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北京 101111
Quick Overview for TLR4 抗体 (ABIN2689940)
抗原
See all TLR4 抗体适用
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产品特性
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The monoclonal antibody HTA125 reacts with the human Toll-like receptor 4 (TLR4). Toll-like receptors (TLR) are type I transmembrane proteins characterized by an extracellular domain containing leucine-rich repeats and a cytoplasmic domain similar to IL-1R family. In human, ten TLR have been identified so far. The TLR family acts as pattern-recognition receptor is the essential component of pathogen recognition and innate immunity. TLR4 has been identified as the receptor for Gram-negative bacterial LPS. TLR4 mRNA has been found in spleen, placenta, ovary, intestine and lung. Using HTA125, TLR4 protein expression has been detected in monocytes, B cells, and T cells in human peripheral blood. Human TLR4 gene has been mapped to chromosome 9q32. The immunogen used to generate HTA125 hybridoma was human TLR4-transfected Ba/F3 cell line. Detection of TLR4 expression on human peripheral monocytes by purified anti-human TLR4 antibody HTA125 (Cat. No. 551964). Human PBMC were stained with 0.5 μg of purified HTA125 using 3-step staining protocol outlined below and anti-human CD14-FITC (Cat. No. 555397) and analyzed with a BD FACScan™ Flow Cytometer (Becton Dickinson, San Jose, CA).
BD Pharmingen™ Purified Mouse Anti-Human Toll-Like Receptor 4 - Purified - Clone HTA125 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human TLR4-transfected Ba/F3 cell line
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亚型
- IgG2a kappa
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anti-Toll-Like Receptor 4 (TLR4) (AA 751-835) antibody
TLR4 适用: 人, 小鼠, 大鼠, Cow WB, FACS, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibodyTLR4 适用: 人 WB, FACS, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 49-247) antibodyTLR4 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (HRP)TLR4 适用: 人 WB, FACS, IHC, ICC, IF 宿主: 兔 Polyclonal HRP
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (FITC)TLR4 适用: 人 WB, FACS, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Biotin)TLR4 适用: 人 WB, FACS, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PE)TLR4 适用: 人 WB, FACS, IHC, ICC, IF 宿主: 兔 Polyclonal PE
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (APC)TLR4 适用: 人 WB, FACS, IHC, ICC, IF 宿主: 兔 Polyclonal APC
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PerCP)TLR4 适用: 人 WB, FACS, IHC, ICC, IF 宿主: 兔 Polyclonal PerCP
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Atto 488)TLR4 适用: 人 WB, FACS, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Toll-like receptor 4 mediates inflammatory signaling by bacterial lipopolysaccharide in human hepatic stellate cells." in: Hepatology (Baltimore, Md.), Vol. 37, Issue 5, pp. 1043-55, (2003) (PubMed).
: "Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 2, pp. 554-61, (2002) (PubMed).
: "Toll-like receptors and innate immunity." in: Advances in immunology, Vol. 78, pp. 1-56, (2001) (PubMed).
: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, (2001) (PubMed).
: "Tlr4: central component of the sole mammalian LPS sensor." in: Current opinion in immunology, Vol. 12, Issue 1, pp. 20-6, (2000) (PubMed).
: "Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2." in: Biochemical and biophysical research communications, Vol. 268, Issue 1, pp. 172-7, (2000) (PubMed).
: "Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9." in: European cytokine network, Vol. 11, Issue 3, pp. 372-8, (2000) (PubMed).
: "Three novel mammalian toll-like receptors: gene structure, expression, and evolution." in: European cytokine network, Vol. 11, Issue 3, pp. 362-71, (2000) (PubMed).
: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, (1999) (PubMed).
: "TLR6: A novel member of an expanding toll-like receptor family." in: Gene, Vol. 231, Issue 1-2, pp. 59-65, (1999) (PubMed).
: "A family of human receptors structurally related to Drosophila Toll." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 588-93, (1998) (PubMed).
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: "Toll-like receptor 4 mediates inflammatory signaling by bacterial lipopolysaccharide in human hepatic stellate cells." in: Hepatology (Baltimore, Md.), Vol. 37, Issue 5, pp. 1043-55, (2003) (PubMed).
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- TLR4 (Toll-Like Receptor 4 (TLR4))
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别名
- Toll-Like Receptor 4
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背景
- Synonyms: TLR4, CD284
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins
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