Nkx2-2 抗体
(5 references)-
- 抗原 See all Nkx2-2 抗体
- Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))
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适用
- 人, 小鼠, 大鼠, 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nkx2-2 antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), BioImaging (BI), Intracellular Staining (ICS)
- 品牌
- BD Pharmingen™
- 产品特性
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The 74.5A5 monoclonal antibody specifically binds to Homeobox protein Nkx-2.2 (Nkx2.2), which is a transcription factor required for the differentiation of ventral neuronal populations in the central nervous system and endocrine cell populations in the pancreas and intestine. Within the pancreas, Nkx2.2 is expressed in many cell subsets and plays important roles in islet and β-cell differentiation and maturation. Mice with a homozygous disruption of the Nkx2-2 gene lack mature β cells and die shortly after birth with severe hyperglycemia. Similarly to the Nkx2.2-ablated mice, homozygous mutations in the human NKX2-2 gene have been described in diabetic patients and associated with the etiology of neonatal diabetes. The NKX2-2 gene is also a target of the Ewing sarcoma specific protein EWS-FLI. Nkx2.2 expression can be monitored in order to determine cell fate changes in cultures for the generation of human pluripotent stem cell-derived insulin-producing cells as well as oligodendrocyte progenitors. LEFT: Western blot analysis of Nkx2.2 expression in human embryonic kidney cell lysate. Lysates of 293-F cells (Life Technologies R790-07) were prepared for electrophoresis (SDS-PAGE) in a 2D Tris-Glycine polyacrylamide gel. The proteins were transferred to PVDF membranes and then probed with 0.5, 0.25, 0.125, 0.062 μg/mL (Lanes 1-4, respectively) of Purified Mouse Anti-Nkx2.2. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). RIGHT: Flow cytometric analysis of Nkx2.2 expression in human embryonic kidney cells. 293-F cells (Life Technologies R790-07) were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574). The fixed cells were then stained with either Purified Mouse IgG2b, Isotype Control (Cat. No. 556654, dashed line) or Purified Mouse Anti-Nkx2.2 (solid line). Specific staining was detected with PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System. LEFT: Immunohistochemical staining Nkx2.2 in human pancreas. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG2b κ Isotype Control (Cat. No.556654, left) or Purified Mouse Anti-Nkx2.2 (right). A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x. RIGHT: Immunofluorescent staining of Nkx2.2 in mouse insulinoma cells. Beta-TC-6 cells (ATCC CRL-11506) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050), and stained with Purified Mouse Anti-Nkx2.2 at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 Goat Anti-Mouse Ig (Life Technologies, pseudo-colored green), and the counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software. Permeabilization with BD Perm/Wash™ Buffer (Cat No. 554723) or 0.1 % Triton™ X-100 buffer is also suitable for use with this antibody. 564731 Rev. 1 Page 1 of 2
BD Pharmingen™ Purified Mouse Anti-Nkx2.2 - Purified - Clone 74.5A5 - Isotype Mouse IgG2b, κ - Reactivity Hu, Ms, Chick, Rat - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Chicken Nkx2.2 Recombinant Protein
- 克隆位点
- 74-5A5
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product Nkx2-2 Primary Antibody
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anti-NK2 Homeobox 2 (Nkx2-2) (AA 1-119) antibody
Nkx2-2 适用: 人 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal NX2-1524 unconjugated
anti-NK2 Homeobox 2 (Nkx2-2) (AA 1-119) antibodyNkx2-2 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal NX2-1523 unconjugated
anti-NK2 Homeobox 2 (Nkx2-2) antibodyNkx2-2 适用: 人, 小鼠, 大鼠, 小鸡 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal NX2-294 unconjugated
anti-NK2 Homeobox 2 (Nkx2-2) antibodyNkx2-2 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3-00E-04 unconjugated
anti-NK2 Homeobox 2 (Nkx2-2) (N-Term) antibodyNkx2-2 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 豚鼠, 马, 绵羊 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-NK2 Homeobox 2 (Nkx2-2) antibodyNkx2-2 适用: 人, 小鼠, 大鼠, 小鸡 IHC, StM 宿主: 小鼠 Monoclonal rNX2-294 unconjugated Recombinant Antibody
anti-NK2 Homeobox 2 (Nkx2-2) antibodyNkx2-2 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal SPM564 unconjugated
anti-NK2 Homeobox 2 (Nkx2-2) (AA 1-119) antibodyNkx2-2 适用: 人 IHC, StM 宿主: 兔 Monoclonal NX2-2198R unconjugated
anti-NK2 Homeobox 2 (Nkx2-2) antibodyNkx2-2 适用: 人 IHC, StM 宿主: 兔 Monoclonal NX2-1422R unconjugated Recombinant Antibody
anti-NK2 Homeobox 2 (Nkx2-2) (AA 1-119) antibodyNkx2-2 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rNX2-1523 unconjugated Recombinant Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "NKX2.2 is a useful immunohistochemical marker for Ewing sarcoma." in: The American journal of surgical pathology, Vol. 36, Issue 7, pp. 993-9, (2012) (PubMed).
: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells." in: Nature biotechnology, Vol. 24, Issue 11, pp. 1392-401, (2006) (PubMed).
: "Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling." in: Nature, Vol. 398, Issue 6728, pp. 622-7, (1999) (PubMed).
: "Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells." in: Development (Cambridge, England), Vol. 125, Issue 12, pp. 2213-21, (1998) (PubMed).
: "Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling." in: Cell, Vol. 90, Issue 1, pp. 169-80, (1997) (PubMed).
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: "NKX2.2 is a useful immunohistochemical marker for Ewing sarcoma." in: The American journal of surgical pathology, Vol. 36, Issue 7, pp. 993-9, (2012) (PubMed).
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- 抗原
- Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))
- 别名
- Nkx2.2 (Nkx2-2 产品)
- 别名
- XeNK-2 antibody, Xnkx2.2 antibody, nkx2-2-A antibody, nkx2.2 antibody, NKX-2.2 antibody, NKX2.2 antibody, NKX2B antibody, Nkx-2.2 antibody, Nkx2.2 antibody, tinman antibody, id:ibd5090 antibody, nk2.2 antibody, nkx2-2 antibody, wu:fc83b01 antibody, zgc:136536 antibody, NK2 homeobox 2 S homeolog antibody, NK2 homeobox 2 antibody, NK2 homeobox 2a antibody, nkx2-2.S antibody, NKX2-2 antibody, Nkx2-2 antibody, nkx2.2a antibody
- 背景
- Synonyms: NKX2-2, NKX2B, Nkx2-2, Nkx-2.2, NK-2 homolog B, glycoprotein GP330
- 分子量
- 30-40 kDa
- 途径
- Dopaminergic Neurogenesis
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