(2 references)-
- 抗原 See all IL-7 (IL7) 抗体
- IL-7 (IL7) (Interleukin 7 (IL7))
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适用
- 人
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-7 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- 品牌
- BD Pharmingen™
- 产品特性
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Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) reacts with human interleukin-7 (IL-7). This antibody can be useful as a detection antibody for sandwich ELISA measuring human IL-7 protein levels. Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10) can be paired with Purified Rat Anti-Human IL-7 antibody (clone BVD10-40F6, Cat. No. 554493) as the capture antibody along with recombinant human IL-7 (Cat. No. 554608) as the standard. Investigators are advised to titrate to determine optimal usage concentrations (ELISA), with a suggested range of 2 μg/mL to 0.5 μg/mL for Biotin Rat Anti-Human IL-7 antibody (clone BVD10-11C10). To obtain linear standard curves, doubling dilutions of human IL-7 ranging from ~2,000 to 15 pg/mL are recommended for inclusion in each ELISA plate. This ELISA pairing has been reported to demonstrate limited to no cross-reactivity with the following cytokines: Mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF or Human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL- 9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF, rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF.
BD Pharmingen™ Biotin Rat Anti-Human IL-7 - Biotin - Clone BVD10-11C10 - Isotype Rat IgG2a - Reactivity Hu - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Recombinant Human IL-7
- 克隆位点
- BVD10-11C10
- 亚型
- IgG2a
- Top Product
- Discover our top product IL7 Primary Antibody
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anti-Interleukin 7 (IL7) (AA 1-177) antibody
IL7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) antibodyIL7 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 51-154) antibodyIL7 适用: 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 7 (IL7) antibodyIL7 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (C-Term) antibodyIL7 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) antibody (Biotin)IL7 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 7 (IL7) antibody (Biotin)IL7 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
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- 抗原
- IL-7 (IL7) (Interleukin 7 (IL7))
- 别名
- IL-7 (IL7 产品)
- 别名
- IL7 antibody, IL-7 antibody, A630026I06Rik antibody, Il-7 antibody, hlb368 antibody, interleukin 7 antibody, IL7 antibody, Il7 antibody
- 途径
- JAK/STAT Signaling
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