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IKZF1 抗体

Name: IKZF1 抗体
SKU: ABIN2689714
Availability: OutOfStock

IKZF1 适用: 人, 小鼠 WB, ICS 宿主: 小鼠 Monoclonal R32 unconjugated

(5 references)

产品编号 ABIN2689714

发货至: 中国

Datasheet as PDF

抗原 See all IKZF1 抗体

IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))
适用
58

39

17

5

5

4

4

4

4

1

1

1

1
人, 小鼠
宿主
61

11

2
小鼠
Compatible Secondaries
克隆类型
62

12
单克隆
标记
37

5

5

4

4

2

2

2

2

2

2

2

1

1

1

1

1
This IKZF1 antibody is un-conjugated
应用范围
60

25

20

19

13

13

10

8

7

4

3

3

2

1

1

1
Western Blotting (WB), Intracellular Staining (ICS)

品牌

BD Pharmingen™

产品特性

The R32-1149 monoclonal antibody specifically binds to Ikaros, which is encoded by IKZF1 (IKAROS family zinc finger 1). Ikaros is also known as Lymphoid transcription factor LyF-1 (LYF1) and ZNFN1A1 [Zinc finger protein, subfamily 1A, 1 (Ikaros)]. Ikaros belongs to the IKAROS family of zinc-finger transcription factors. This DNA-binding transcription factor forms homodimers and heterodimers with other IKAROS family members. It is involved in the development of lymphoid tissues and remains abundantly expressed in the thymus and by cells within peripheral lymphoid tissues. Ikaros functions in the maturation, differentiation, and homeostasis of various hematopoietic cells including T lymphocytes, B cells, NK cells, and neutrophils. Ikaros regulates the expression of a number of genes including various genes expressed during early stages of B- and T-cell development. Several alternatively-spliced human Ikaros isoforms have been described. Abnormal expression of different Ikaros isoforms has been associated with hematologic malignancies. Flow cytometric analysis of 293F cells transfected with members of the IKAROS family has demonstrated that clone R32-1149 is negative on Helios, Aiolos, and Eos transfectants. Analysis of Ikaros Expression Left Panel - Flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear (PBMC) cells were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). The cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line histogram) or Purified Mouse Anti-Ikaros antibody (Cat. No. 564475, solid line histogram). The cells were washed and stained with PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Middle Panel - Western blot analysis of Ikaros expressed by human PBMC. A PBMC lysate was electrophoresed (SDS-PAGE) and the cellular proteins were transferred to a membrane where they were probed using Purified Mouse Anti-Ikaros antibody at concentrations of 4 (lane 1), 1 (lane 2), and 0.25 μg/mL (lanes 3). Ikaros was identified in bands ranging in size from ~50-70 kDa. Right Panel - Ikaros staining of human tonsil. Tissue sections were prepared from formalin-fixed paraffin-embedded tonsils. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the sections were stained with either Purified Mouse IgG1 κ Isotype Control (Left Tonsil Section, 20x original) or Purified Mouse Anti-Ikaros antibody (Middle and Right Tonsil sections at 20x and 40x original, respectively). A three-step staining procedure that employs a Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The tissue was counterstained with Hematoxylin. Ikaros staining is detected in the nuclei of some lymphocytes.

BD Pharmingen™ Purified Mouse Anti-Human Ikaros - Purified - Clone R32-1149 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 50 μg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human Ikaros Recombinant Protein

克隆位点

R32

亚型

IgG1 kappa

Top Product

Discover our top product IKZF1 Primary Antibody
anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 1-265) antibody
IKZF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 1-270) antibody
IKZF1 适用: 人 WB, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 415-444), (C-Term) antibody
IKZF1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB24656 unconjugated

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (C-Term) antibody (PE)
IKZF1 适用: 人, 小鼠 FACS 宿主: 小鼠 Monoclonal 4E9 PE

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) antibody
IKZF1 适用: 人 WB, IF, ChIP 宿主: 兔 Monoclonal unconjugated

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 428-459), (C-Term) antibody
IKZF1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (Middle Region) antibody
IKZF1 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (AA 1-477) antibody
IKZF1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (Internal Region) antibody
Verified IKZF1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1) (N-Term) antibody
IKZF1 适用: 小鼠 WB, ChIP, ChIP-seq 宿主: 小鼠 Monoclonal 2A9 unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Yoshida, Landhuis, Dose, Hazan, Zhang, Naito, Jackson, Wu, Perotti, Kaufmann, Gounari, Morgan, Georgopoulos: "Transcriptional regulation of the Ikzf1 locus." in: Blood, Vol. 122, Issue 18, pp. 3149-59, (2013) (PubMed).

Georgopoulos: "Haematopoietic cell-fate decisions, chromatin regulation and ikaros." in: Nature reviews. Immunology, Vol. 2, Issue 3, pp. 162-74, (2002) (PubMed).

Westman, Mackay, Gell: "Ikaros: a key regulator of haematopoiesis." in: The international journal of biochemistry & cell biology, Vol. 34, Issue 10, pp. 1304-7, (2002) (PubMed).

Nietfeld, Meyerhans: "Cloning and sequencing of hIk-1, a cDNA encoding a human homologue of mouse Ikaros/LyF-1." in: Immunology letters, Vol. 49, Issue 1-2, pp. 139-41, (1996) (PubMed).

Molnár, Wu, Largespada, Vortkamp, Scherer, Copeland, Jenkins, Bruns, Georgopoulos: "The Ikaros gene encodes a family of lymphocyte-restricted zinc finger DNA binding proteins, highly conserved in human and mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 2, pp. 585-92, (1996) (PubMed).
抗原

IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

别名

Ikaros (IKZF1 产品)

别名

Hs.54452 antibody, IK1 antibody, IKAROS antibody, LYF1 antibody, PRO0758 antibody, ZNFN1A1 antibody, hIk-1 antibody, RGD1562979 antibody, ikaros antibody, znfn1a1 antibody, ik1 antibody, lyf1 antibody, hik-1 antibody, pro0758 antibody, hs.54452 antibody, MGC108252 antibody, 5832432G11Rik antibody, Ikaros antibody, LyF-1 antibody, Zfpn1a1 antibody, Znfn1a1 antibody, hlk-1 antibody, mKIAA4227 antibody, ikzf1 antibody, IKAROS family zinc finger 1 antibody, IKAROS family zinc finger 1 (Ikaros) antibody, IKZF1 antibody, Ikzf1 antibody, ikzf1 antibody

背景

Synonyms: IKZF1, LYF1, hIk-1, IK1, ZNFN1A1

途径

Production of Molecular Mediator of Immune Response