Interferon gamma 抗体
Quick Overview for Interferon gamma 抗体 (ABIN967468)
抗原
See all Interferon gamma (IFNG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- Purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Partially purified human IFN-gamma from supernatants of human PBMC stimulated with Staphylococcus aureus
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亚型
- IgG1 kappa
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anti-Interferon gamma (IFNG) (AA 75-155) antibody
IFNG 适用: 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 81-166) antibodyIFNG 适用: 人 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 23-156) antibodyIFNG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) antibody (Biotin)IFNG 适用: 人, Non-Human Primate WB, ELISA, FACS, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal 4S-B3 Biotin
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IFNG-466 unconjugated
anti-Interferon gamma (IFNG) antibodyMicroarray verified IFNG 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB393378 unconjugated
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人, Non-Human Primate WB, ELISA, FACS, IP, ICC, IHC (p), RIA 宿主: 小鼠 Monoclonal 4S-B3 unconjugated
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal F2E4 unconjugated
anti-Interferon gamma (IFNG) antibody (APC)IFNG 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal XMG1-2 APC
anti-Interferon gamma (IFNG) (AA 24-166) antibodyIFNG 适用: 豚鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D1 unconjugated
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应用备注
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Blocking Control for Intracellular Staining:
The purified 4S.B3 antibody (ABIN967468) can be used as a blocking control to demonstrate specificity of IFN-gamma staining by directly conjugated clone 4S.B3. To perform this control, the fixed/permeabilized cells (~1 million) can be incubated with 1-10 µg of purified 4S.B3 antibody (ABIN967468) for 20 minutes at 4°C, prior to staining with the directly conjugated antibody (e.g., 0.1 -0.5 µg mAb/1 million cells) (right panel). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
ELISA Detection:
The biotinylated 4S.B3 antibody is useful as a detection antibody in a sandwich ELISA for measuring human IFN-gamma protein levels. Biotinylated 4S.B3 antibody can be paired with the purified NIB42 antibody as the capture antibody, with recombinant human IFN-gamma as the standard. For testing human IFN-gamma complex in biological fluids like serum or plasma, our human IFN-gamma specific OptEIA™ sandwich ELISA set and OptEIA™ sandwich ELISA kit are recommended.
Western Blot:
The 4S.B3 antibody has been found useful for Western blotting. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/ml
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缓冲液
- Aqueous buffered solution.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Interferon gamma (IFNG)
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别名
- IFN-gamma
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背景
- The 4S.B3 antibody reacts with human interferon-gamma (IFN-gamma). The immunogen used to generate this hybridoma was partially purified human IFN-gamma obtained from supernatants of human PBMC stimulated with Staphylococcus aureus. This is a neutralizing antibody.
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
抗原
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