(6 references)-
- 抗原 See all GZMA 抗体
- GZMA (Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GZMA antibody is un-conjugated
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应用范围
- Immunoprecipitation (IP), Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The primary mechanism by which cytotoxic T cells eliminate virally infected cells is by granule exocytosis. The release of cytotoxic granule contents by cytotoxic T lymphocytes (CTL) triggers apoptotic target cell death. CTL granules contain a pore-forming protein, perforin, and a group of serine proteases called granzymes. In the classic model, perforins create holes in the target cell membrane, allowing entrance of the granzymes. Granzyme A and B are the predominant granzymes activated after CTL activation, but each act via an independent apoptotic pathway, granzyme B is activated immediately, while granzyme A acts hours later. Granzyme B has been shown to induce apoptosis and to cleave a number of substrates which are similar in specificity to those of the caspase family of proteinases. Granzyme B can cleave substrates, such as DNA-PKcs, and nuclear mitotic apparatus protein (NuMA). Furthermore, Granzyme B can also cleave substrates such as Bid and DFF45 in a caspase-independent fashion. Studies involving mice which are deficient in both granzyme A and B suggest a model whereby the granzyme B pathway may have evolved as the major apoptotic pathway with the granzyme A pathway acting as a backup. However, further research is needed to delineate the components of these distinct pathways.
BD Pharmingen™ Purified Mouse Anti-Human Granzyme A - Purified - Clone CB9 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- CB9
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product GZMA Primary Antibody
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anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) antibody
GZMA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) antibodyGZMA 适用: 人 IP, FACS, ICC 宿主: 小鼠 Monoclonal CB9 unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (AA 29-262) antibodyGZMA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) antibodyGZMA 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (AA 101-200) antibodyGZMA 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (AA 29-260) antibodyGZMA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (AA 29-262) antibodyGZMA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (AA 29-260) antibodyGZMA 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (AA 41-141) antibodyGZMA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4C6 unconjugated
anti-Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA) (C-Term) antibodyGZMA 适用: 人, 大鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Granzyme B short-circuits the need for caspase 8 activity during granule-mediated cytotoxic T-lymphocyte killing by directly cleaving Bid." in: Molecular and cellular biology, Vol. 20, Issue 11, pp. 3781-94, (2000) (PubMed).
: "Granzyme A loading induces rapid cytolysis and a novel form of DNA damage independently of caspase activation." in: Immunity, Vol. 10, Issue 5, pp. 585-94, (1999) (PubMed).
: "Granzyme A initiates an alternative pathway for granule-mediated apoptosis." in: Immunity, Vol. 10, Issue 5, pp. 595-605, (1999) (PubMed).
: "Granzyme B directly and efficiently cleaves several downstream caspase substrates: implications for CTL-induced apoptosis." in: Immunity, Vol. 8, Issue 4, pp. 451-60, (1998) (PubMed).
: "Circulating CD8 T lymphocytes in human immunodeficiency virus-infected individuals have impaired function and downmodulate CD3 zeta, the signaling chain of the T-cell receptor complex." in: Blood, Vol. 91, Issue 2, pp. 585-94, (1998) (PubMed).
: "Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 17, pp. 9285-90, (1997) (PubMed).
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: "Granzyme B short-circuits the need for caspase 8 activity during granule-mediated cytotoxic T-lymphocyte killing by directly cleaving Bid." in: Molecular and cellular biology, Vol. 20, Issue 11, pp. 3781-94, (2000) (PubMed).
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- 抗原
- GZMA (Granzyme A (Granzyme 1, Cytotoxic T-Lymphocyte-Associated serine Esterase 3) (GZMA))
- 别名
- Granzyme A (GZMA 产品)
- 别名
- gzmA antibody, GZMA antibody, AW494114 antibody, Ctla-3 antibody, Ctla3 antibody, Hf antibody, SE1 antibody, TSP-1 antibody, TSP1 antibody, CTLA3 antibody, HFSP antibody, granzyme A antibody, Granzyme A antibody, granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) antibody, gzmA antibody, GZMA antibody, graa antibody, Gzma antibody
- 途径
- Apoptosis
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