EPH Receptor B2 抗体
(4 references)-
- 抗原 See all EPH Receptor B2 (EPHB2) 抗体
- EPH Receptor B2 (EPHB2)
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This EPH Receptor B2 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
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The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2). EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors. EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B). The EphB2 receptor can bind to ephrin-B1, ephrin-B2, and ephrin-B3. Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems. The EphB2 receptor functions as a chemodirectant in regulating cellular migration. EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, thereby influencing cell fate, morphogenesis and organogenesis. Signaling can occur in a forward pathway when the EphB2 receptor tyrosine kinase is activated by bound ligand and in a reverse pathway when transmembrane ephrin-B ligands are activated by EphB2 receptor-mediated crosslinking. In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions such as cancer. Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation. Ephrin-B2 Molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes. This interaction promotes monocyte differentiation into proinflammatory macrophages. In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression. The differential expression patterns of EphB2 allows for the detection and isolation of various intestinal epithelial cell types. These include intestinal stem cells (ISCs) which express high levels of EphB2. The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2. Flow cytometric analysis of EphB2 expression on human Colo-205 cells. Cells from the human COLO 205 (Colorectal adenocarcinoma, ATCC CCL-222) cell line were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 550878, dashed line histogram) or Purified Mouse Anti-Human EphB2 antibody (Cat. No. 564698, solid line histogram). The cells were washed and counterstained with PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms showing EphB2 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Immunohistochemical analysis of human EphB2 expression in human placenta. Acetone-fixed frozen human placenta sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat No.550878, Left Image) or Purified Mouse Anti-Human EphB2 antibody (Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification:20x. 564698 Rev. 1 Page 1 of 2
BD Pharmingen™ Purified Mouse Anti-Human EphB2 - Purified - Clone 2H9 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human EphB2 Recombinant Protein
- 克隆位点
- 2H9
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product EPHB2 Primary Antibody
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anti-EPH Receptor B2 (EPHB2) antibody
EPHB2 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 48CT12-6-4 unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 551-650) antibodyEPHB2 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 226-325) antibodyEPHB2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D1 unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 17-200) antibodyEPHB2 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 2D12C6 unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 1021-1050), (C-Term) antibodyEPHB2 适用: 人 WB, IHC (p), IF 宿主: 兔 Polyclonal RB01652 unconjugated
anti-EPH Receptor B2 (EPHB2) (AA 261-465) antibodyEPHB2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (C-Term) antibodyEPHB2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (pTyr298) antibodyEPHB2 适用: 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (pTyr317) antibodyEPHB2 适用: 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor B2 (EPHB2) (pTyr331) antibodyEPHB2 适用: 小鸡 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Isolation and in vitro expansion of human colonic stem cells." in: Nature medicine, Vol. 17, Issue 10, pp. 1225-7, (2011) (PubMed).
: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).
: "EphB-ephrin-B2 interactions are required for thymus migration during organogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 30, pp. 13414-9, (2010) (PubMed).
: "EphB2 as a therapeutic antibody drug target for the treatment of colorectal cancer." in: Cancer research, Vol. 64, Issue 3, pp. 781-8, (2004) (PubMed).
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: "Isolation and in vitro expansion of human colonic stem cells." in: Nature medicine, Vol. 17, Issue 10, pp. 1225-7, (2011) (PubMed).
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- 抗原
- EPH Receptor B2 (EPHB2)
- 别名
- EphB2 (EPHB2 产品)
- 别名
- CAPB antibody, DRT antibody, EK5 antibody, EPHT3 antibody, ERK antibody, Hek5 antibody, PCBC antibody, Tyro5 antibody, ERK2 antibody, ERT1 antibody, MAPK2 antibody, P42MAPK antibody, PRKM1 antibody, PRKM2 antibody, p38 antibody, p40 antibody, p41 antibody, p41mapk antibody, RGD1564232 antibody, Cek5 antibody, Drt antibody, ETECK antibody, Erk antibody, Nuk antibody, Prkm5 antibody, Qek5 antibody, Sek3 antibody, cek5 antibody, ephb2 antibody, xEphB2 antibody, si:ch73-160e9.1 antibody, tpx2 antibody, wu:fb09e10 antibody, wu:fc64a03 antibody, wu:fi18g12 antibody, zgc:112296 antibody, EPH receptor B2 antibody, mitogen-activated protein kinase 1 antibody, Eph receptor B2 antibody, EPH receptor B2 L homeolog antibody, eph receptor B2a antibody, eph receptor B2b antibody, EPHB2 antibody, MAPK1 antibody, Ephb2 antibody, ephb2.L antibody, ephb2a antibody, ephb2b antibody
- 背景
- Synonyms: EPHB2, EphB2R, TYRO5, CAPB, DRT, EK5, EPHT3, EPTH3, HEK5, PCBC
- 途径
- RTK signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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