CD81 抗体 (Biotin)
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北京 101111
Quick Overview for CD81 抗体 (Biotin) (ABIN2689414)
抗原
See all CD81 抗体适用
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克隆位点
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产品特性
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The Eat2 antibody reacts with CD81, a 26- kDa nonglycosylated member of the transmembrane 4 integral membrane protein superfamily, expressed by many types of cells. For example, CD81 participates with CD19 and CD21 in the signal transduction complex associated with the B-cell receptor on human B lymphocytes and with the CD4 and CD8 co-receptors on human thymocytes and T lymphocytes. In mouse fetal thymic organ culture, interactions of immature thymocytes with CD81 expressed by thymic stromal cells are required to induce development of T cells with αβ T-cell receptors. Furthermore, CD81 has been shown to play a role in the regulation of rat mastcell degranulation. Despite its important roles in the immune response and wide tissue distribution, CD81-deficient mice are born without obvious developmental abnormalities. However, these mice have abnormal immune responses, and impaired fertility. Eat2 mAb cross-reacts with the rat CD81 antigen. Two-color analysis of the expression of CD81 on mouse and rat splenocytes. C57BL/6 splenocytes were simultaneously stained with FITC Rat anti-Mouse CD45R/B220 (Cat. no. 553087/553088, First and second panels) and PE Hamster IgG1 κ Isotype Control (Cat. no. 553972, First panel) or PE Hamster Anti-Mouse/Rat CD81 (Cat. No, 559519, Second panel) monoclonal antibodies. Similarly, LOU splenocytes were simultaneously stained with FITC Mouse anti-Rat CD45RA (Cat. No. 554883, Third and last panels) and PE Hamster IgG1 κ Isotype Control (Cat. no. 553972, Third panel) or PE Hamster anti-Mouse/Rat CD81 (Cat. No, 559519, Last panel) monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ Flow Cytometry System (BD Biosciences,San Jose, CA
BD Pharmingen™ Biotin Hamster Anti-Mouse/Rat CD81 - Biotin - Clone Eat2 - Isotype Armenian Hamster IgG, κ - Reactivity Ms, Rat - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- CD81+ mouse B lymphoma 38C13
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亚型
- IgG kappa
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anti-CD81 (CD81) antibody
KO Validated CD81 适用: 人 FACS, WB, IF, ICC, IHC (p), BP 宿主: 兔 Polyclonal unconjugated
anti-CD81 (CD81) (AA 1-100) antibodyCD81 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD81 (CD81) (AA 190-201), (Extracellular) antibodyCD81 适用: 人 FACS, WB, IHC, LCI 宿主: 兔 Polyclonal unconjugated
anti-CD81 (CD81) antibodyCD81 适用: 人 FACS, WB, IHC, ELISA 宿主: 兔 Monoclonal 9F7 unconjugated Recombinant Antibody
anti-CD81 (CD81) antibodyCD81 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, IF, Func, StM 宿主: 小鼠 Monoclonal 1-3-3-22 unconjugated
anti-CD81 (CD81) antibodyCD81 适用: 人, 小鼠, 大鼠 ELISA, Coat 宿主: 兔 Monoclonal C81-2885R unconjugated Recombinant Antibody
anti-CD81 (CD81) antibodyCD81 适用: 人, 兔, Cat FACS, WB, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal M38 unconjugated
anti-CD81 (CD81) antibody (FITC)CD81 适用: 人, 兔, Cat FACS 宿主: 小鼠 Monoclonal M38 FITC
anti-CD81 (CD81) antibody (PE)CD81 适用: 人, 兔, Cat FACS 宿主: 小鼠 Monoclonal M38 PE
anti-CD81 (CD81) antibodyCD81 适用: 人 FACS, cELISA 宿主: 大鼠 Monoclonal QV-6A8-S3 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Allergen-induced airway hyperreactivity is diminished in CD81-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 5054-61, (2000) (PubMed).
: "Differential expression of murine CD81 highlighted by new anti-mouse CD81 monoclonal antibodies." in: Hybridoma, Vol. 19, Issue 1, pp. 15-22, (2000) (PubMed).
: "CD81 on B cells promotes interleukin 4 secretion and antibody production during T helper type 2 immune responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 5, pp. 2458-62, (1998) (PubMed).
: "CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system." in: Annual review of immunology, Vol. 16, pp. 89-109, (1998) (PubMed).
: "Negative regulation of Fc epsilon RI-mediated degranulation by CD81." in: The Journal of experimental medicine, Vol. 186, Issue 8, pp. 1307-14, (1997) (PubMed).
: "Normal lymphocyte development but delayed humoral immune response in CD81-null mice." in: The Journal of experimental medicine, Vol. 185, Issue 8, pp. 1505-10, (1997) (PubMed).
: "Normal development but differentially altered proliferative responses of lymphocytes in mice lacking CD81." in: The EMBO journal, Vol. 16, Issue 14, pp. 4217-25, (1997) (PubMed).
: "Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 20, pp. 10844-9, (1997) (PubMed).
: "A role for CD81 in early T cell development." in: Science (New York, N.Y.), Vol. 271, Issue 5246, pp. 198-200, (1996) (PubMed).
: "The CD19/CD21 signal transduction complex of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 437-42, (1994) (PubMed).
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: "Allergen-induced airway hyperreactivity is diminished in CD81-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 5054-61, (2000) (PubMed).
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- CD81
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别名
- CD81
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背景
- Synonyms: TAPA-1, Tspan28, Tapa1, Tapa-1, Target of the antiproliferative antibody 1,
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途径
- Inositol Metabolic Process, Hepatitis C
抗原
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