CD80 抗体
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北京 101111
Quick Overview for CD80 抗体 (ABIN2689409)
抗原
See all CD80 抗体适用
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克隆位点
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产品特性
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The 1G10 antibody reacts with CD80 (B7-1). This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell costimulation via interactions with CD28 and CD152 (CTLA-4). CD80 is constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages, and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 is greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours. The 1G10 antibody blocks binding of CTLA-4-Ig to CD80, but it does not block stimulation of T cells by natural antigen-presenting cells. Preliminary evidence has shown that mAb 16-10A1 (Cat. no. 553766) blocks binding of 1G10 mAb to CD80, indicating that the two antibodies may recognize overlapping epitopes on the CD80 Molecule. However, the 16-10A1 mAb recognizes an upregulated antigen on UV-irradiated P815 mastocytoma cells which is not detected by the 1G10 mAb, the cause and significance of this differential reactivity of the two anti-CD80 antibodies is unknown. Expression of membrane CD80 (B7-1) by mouse splenocytes. Freshly isolated (left) or 72-hour LPS-stimulated BALB/c splenocytes (right) were stained with purified 1G10/B7 mAb (open histograms) or no primary mAb (shaded histograms). Staining was detected with biotinylated goat anti-rat Ig (Cat. No. 554014) followed by Streptavidin-PE (Cat. No. 554061). Viable resting lymphocytes (left) and blasts (right) were selected by scatter profile and dye exclusion (BD Pharmingen™ Via-Probe™, Cat. No. 555816/555815). Flow cytometry was performed on a FACScan™ .
BD Pharmingen™ Purified Rat Anti-Mouse CD80 - Purified - Clone 1G10/B7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Activated Mouse 5C2 Cells
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亚型
- IgG2a kappa
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anti-CD80 (CD80) (AA 35-288) antibody
CD80 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 1G3D7 unconjugated
anti-CD80 (CD80) (AA 251-321) antibodyCD80 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD80 (CD80) antibodyCD80 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Monoclonal 3C3 unconjugated Recombinant Antibody
anti-CD80 (CD80) antibodyCD80 适用: 小鼠, 犬 FACS, IP, IHC (fro) 宿主: 仓鼠 Monoclonal 16-10A1 unconjugated
anti-CD80 (CD80) (Extracellular Domain) antibodyCD80 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-233 unconjugated
anti-CD80 (CD80) (Extracellular Domain) antibody (FITC)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 FITC
anti-CD80 (CD80) (Extracellular Domain) antibody (PerCP)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 PerCP
anti-CD80 (CD80) (Extracellular Domain) antibody (PE)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 PE
anti-CD80 (CD80) (Extracellular Domain) antibody (APC)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 APC
anti-CD80 (CD80) antibodyCD80 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 2A2 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Melphalan and other anticancer modalities up-regulate B7-1 gene expression in tumor cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 12, pp. 6230-6, (2000) (PubMed).
: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).
: "Blockade of the CD28 co-stimulatory pathway: a means to induce tolerance." in: Current opinion in immunology, Vol. 6, Issue 5, pp. 797-807, (1995) (PubMed).
: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, (1994) (PubMed).
: "Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression." in: Nature, Vol. 360, Issue 6401, pp. 266-8, (1992) (PubMed).
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: "Melphalan and other anticancer modalities up-regulate B7-1 gene expression in tumor cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 12, pp. 6230-6, (2000) (PubMed).
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- CD80
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别名
- CD80
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背景
- Synonyms: B7-1
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
抗原
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