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CD80 抗体

Name: CD80 抗体
SKU: ABIN2689407
Availability: OutOfStock

This anti-CD80 antibody is a Armenian Hamster 单克隆 antibody detecting CD80 in FACS, IP, IHC (fro) 和 BR. Suitable for 小鼠和犬. This Primary Antibody has been cited in 6+ publications.

(6 references)

产品编号 ABIN2689407

发货至: 中国

Quick Overview for CD80 抗体 (ABIN2689407)

抗原

See all CD80 抗体

CD80

适用

小鼠, 犬

宿主

Armenian Hamster

克隆类型

单克隆

标记

This CD80 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

克隆位点

Discover our top product CD80 Primary Antibody

品牌

BD Pharmingen™

产品特性

The 16-10A1 antibody reacts with CD80 (B7-1). This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell co-stimulation via interactions with CD28 and CD152 (CTLA-4). CD80 has been reported to be constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages, and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 has been reported to be greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that the activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours. The 16-10A1 antibody has been reported to block binding of CTLA-4 Ig to CD80 and to block T-cell activation by Con A-elicited peritoneal exudate cells and CD80-transfected cell lines. However, 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells. CD86 (B7-2) is an alternate ligand for CD28 and CD152 (CTLA-4). Preliminary reports indicate that the 16-10A1 mAb may block the binding of rat anti-CD80 mAb clone 1G10 (Cat. No. 553368). In addition, it has been reported that the 16-10A1 antibody may cross-react with an activation antigen expressed on IFN-γ-activated alveolar macrophages of the dog. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Hamster Anti-Mouse CD80 - Purified - Clone 16-10A1 - Isotype Armenian Hamster IgG2, κ - Reactivity Ms, Dog - 0.5 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse CD80 (B7) Transfected Cell Line

亚型

IgG2 kappa
anti-CD80 (CD80) (AA 35-288) antibody
CD80 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 1G3D7 unconjugated

anti-CD80 (CD80) (AA 251-321) antibody
CD80 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD80 (CD80) antibody
CD80 适用: 小鼠, 犬 FACS, IP, IHC (fro) 宿主: 仓鼠 Monoclonal 16-10A1 unconjugated

anti-CD80 (CD80) (Extracellular Domain) antibody
CD80 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-233 unconjugated

anti-CD80 (CD80) (Extracellular Domain) antibody (FITC)
CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 FITC

anti-CD80 (CD80) (Extracellular Domain) antibody (PerCP)
CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 PerCP

anti-CD80 (CD80) (Extracellular Domain) antibody (PE)
CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 PE

anti-CD80 (CD80) (Extracellular Domain) antibody (APC)
CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 APC

anti-CD80 (CD80) antibody
CD80 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 2A2 unconjugated

anti-CD80 (CD80) (AA 111-265) antibody
CD80 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Herold, Vezys, Koons, Lenschow, Thompson, Bluestone: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, (1997) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

Boussiotis, Gribben, Freeman, Nadler: "Blockade of the CD28 co-stimulatory pathway: a means to induce tolerance." in: Current opinion in immunology, Vol. 6, Issue 5, pp. 797-807, (1995) (PubMed).

Harlan, Hengartner, Huang, Kang, Abe, Moreadith, Pircher, Gray, Ohashi, Freeman: "Mice expressing both B7-1 and viral glycoprotein on pancreatic beta cells along with glycoprotein-specific transgenic T cells develop diabetes due to a breakdown of T-lymphocyte unresponsiveness." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 8, pp. 3137-41, (1994) (PubMed).

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, (1994) (PubMed).

Razi-Wolf, Freeman, Galvin, Benacerraf, Nadler, Reiser: "Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 4210-4, (1992) (PubMed).
抗原

CD80

别名

CD80

背景

Synonyms: B7-1

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints