CD63 抗体
(2 references)Quick Overview for CD63 抗体 (ABIN2689370)
抗原
See all CD63 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
品牌
- BD Pharmingen™
-
产品特性
-
The AD1 antibody reacts with a 50-60- kDa protein that is a homolog (87 % ) of human CD63 (melanomaassociated antigen ME491). CD63, a highly glycosylated type-III lysosomal glycoprotein, is a member of the tetraspan transmembrane 4 superfamily (TM4SF) that also includes CD9, CD37, and CD53. In the rat, CD63 is expressed on mast cells and platelets. CD63 is also found on rat embryonic fibroblasts and hepatic cell, along with cultured cell lines of diverse origin (eg, liver, lung, and heart). Expression is upregulated with culture. CD63 is expressed in close proximity to the FcεRI on rat mast cells and has been shown to partially inhibit IgE-mediated histamine release. The function of CD63 has not been fully characterized. The expression of CD63 on rat CD54+ peritoneal lavage cells. Lewis rat peritoneal lavage cells were incubated simultaneously with either purified Mouse IgG1, κ isotype control (Cat. no. 557273, open histogram) or purified AD1 mAb (shaded histogram), followed by biotinylated A85-1 mAb (rat anti-mouse IgG1, Cat. no. 553441) and finally Streptavidin-FITC (Cat. no. 554060) and PE-conjugated 1A29 mAb (anti-rat CD54, Cat. no. 554970). For analysis, a gate was drawn to include CD54+ cells only (gate not shown). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Rat CD63 - Purified - Clone AD1 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- Rat basophilic leukemia cell line
-
亚型
- IgG1 kappa
-
-
anti-CD63 (CD63) (AA 120-175) antibody
CD63 适用: 人, 小鼠, 大鼠, 猴, 犬 FACS, WB, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-CD63 (CD63) antibodyCD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 unconjugated
anti-CD63 (CD63) antibody (Biotin)CD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 Biotin
anti-CD63 (CD63) antibody (APC)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 APC
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 1H1E11 unconjugated
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 10F11E6 unconjugated
anti-CD63 (CD63) antibodyCD63 适用: 人, 小鼠 FACS, WB, IHC, IF, ISt, StM 宿主: 小鼠 Monoclonal MX-49-129-5 unconjugated
anti-CD63 (CD63) antibody (PerCP)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PerCP
anti-CD63 (CD63) antibody (PE)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PE
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1E8D4 unconjugated
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C.
-
-
-
: "Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat." in: Shock (Augusta, Ga.), Vol. 3, Issue 2, pp. 125-31, (1995) (PubMed).
: "The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 862-70, (1992) (PubMed).
-
: "Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat." in: Shock (Augusta, Ga.), Vol. 3, Issue 2, pp. 125-31, (1995) (PubMed).
-
- CD63
-
别名
- CD63
-
背景
- Synonyms: ME491
抗原
-
