CD63 抗体
(2 references)Quick Overview for CD63 抗体 (ABIN2689369)
抗原
See all CD63 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
品牌
- BD Pharmingen™
-
产品特性
-
The NVG-2 monoclonal antibody specifically binds to CD63 which is also known as Tspan30, Melanoma 1 antigen, ME491, and C75951. CD63 is a tetraspanin which belongs to the transmembrane-4 glycoprotein superfamily (TM4SF). It constitutes a major component of lysosomal membranes and translocates to the cell surface in response to various stimuli. CD63 is expressed by activated platelets, granulocytes, monocytes, macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 can associate with other membrane molecules and functions in cellular signaling, adhesion and motility. Analyses of CD63 expression. Panel 1 and 2. Multicolor flow cytometric analysis of CD63 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927, Panel 1) or Purified Rat Anti-Mouse CD63 antibody (Cat. No. 564221, Panel 2). The cells were washed and counterstained with PE Goat Anti-Rat Ig (Cat. No. 550767). After washing, the cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 553129/561083). Two-color flow cytometric dot plots show the correlated expression patterns of Ly-6G and Ly-6C versus CD63 (or Ig Isotype control staining) for gated events with the forward and sid light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Panel 3. Immunofluorescent staining of CD63 in bEnd.3 cells. Adherent bEnd.3 cells (ATCC CRL-2299) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1 % Triton™ X-100 (Sigma, X100), and stained with either Purified Rat IgG2a, κ Isotype Control (Upper Panel 3) or Purified Rat Anti-Mouse CD63 antibody (Lower Panel 3). The cells were washed and then counterstained with Alexa Fluor® 488 Goat Anti-Rat IgG (H+L) Antibody (Life Technologies, Cat. No. A11006, pseudocolored green) and Hoechst 33342 Solution (Cat. No. 561908, pseudocolored blue). Images were analyzed using a BD Pathway™ 435 cell imager and merged using BD AttoVision™ Software.
BD Pharmingen™ Purified Rat Anti-Mouse CD63 - Purified - Clone NVG-2 - Isotype Rat IgG2a, κ - Reactivity Ms - 50 μg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- Mouse Eosinophils
-
亚型
- IgG2a kappa
-
-
anti-CD63 (CD63) (AA 120-175) antibody
CD63 适用: 人, 小鼠, 大鼠, 猴, 犬 FACS, WB, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-CD63 (CD63) antibodyCD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 unconjugated
anti-CD63 (CD63) antibody (Biotin)CD63 适用: 人 FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal MEM-259 Biotin
anti-CD63 (CD63) antibody (APC)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 APC
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 1H1E11 unconjugated
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 10F11E6 unconjugated
anti-CD63 (CD63) antibodyCD63 适用: 人, 小鼠 FACS, WB, IHC, IF, ISt, StM 宿主: 小鼠 Monoclonal MX-49-129-5 unconjugated
anti-CD63 (CD63) antibody (PerCP)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PerCP
anti-CD63 (CD63) antibody (PE)CD63 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-259 PE
anti-CD63 (CD63) (AA 103-203) antibodyCD63 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1E8D4 unconjugated
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C.
-
-
-
: "SIRP?/CD172a regulates eosinophil homeostasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2268-77, (2011) (PubMed).
: "Molecular cloning of the murine homologue of CD63/ME491 and detection of its strong expression in the kidney and activated macrophages." in: Biochimica et biophysica acta, Vol. 1217, Issue 3, pp. 312-6, (1994) (PubMed).
-
: "SIRP?/CD172a regulates eosinophil homeostasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2268-77, (2011) (PubMed).
-
- CD63
-
别名
- CD63
-
背景
- Synonyms: Tspan30, Melanoma 1 antigen, ME491, C75951
抗原
-
