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CD6 抗体

This anti-CD6 antibody is a 小鼠 单克隆 antibody detecting CD6 in FACS, IP, IHC (p) 和 IHC (fro). Suitable for 大鼠. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN2689349
发货至: 中国

Quick Overview for CD6 抗体 (ABIN2689349)

抗原

See all CD6 抗体
CD6

适用

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大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD6 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

OX
  • 品牌

    BD Pharmingen™

    产品特性

    The OX-52 antibody reacts with CD6, a 95-120- kDa antigen on T lymphocytes and thymocytes expressing αβ and γδ Tcell receptors. Rat NK cells express low levels of CD6 and are CD8a+, CD8b-, CD5-, and CD3-. In immunoprecipitation experiments, CD6 has been shown to co-precipitate with CD5 and to play a key role in CD5 tyrosine phosphorylation. Two-color evaluation of splenic T lymphocytes. LEW splenocytes were simultaneously stained with PE-conjugated anti-rat CD3 mAb G4.18 (Cat. No. 554833) and purified mAb OX-52 (right panel), followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

    BD Pharmingen™ Purified Mouse Anti-Rat CD6 - Purified - Clone OX-52 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.1 mg

    纯化方法

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    免疫原

    PVG-RT1c rat thoracic-duct leukocytes and splenocytes

    亚型

    IgG2a kappa
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Aqueous buffered solution containing ≤0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store undiluted at 4°C.
  • Castro, Nunes, Oliveira, Tavares, Simões, Parnes, Moreira, Carmo: "OX52 is the rat homologue of CD6: evidence for an effector function in the regulation of CD5 phosphorylation." in: Journal of leukocyte biology, Vol. 73, Issue 1, pp. 183-90, (2003) (PubMed).

    Lawetzky, Tiefenthaler, Kubo, Hünig: "Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta." in: European journal of immunology, Vol. 20, Issue 2, pp. 343-9, (1990) (PubMed).

    Robinson, Puklavec, Mason: "MRC OX-52: a rat T-cell antigen." in: Immunology, Vol. 57, Issue 4, pp. 527-31, (1986) (PubMed).

  • 抗原

    CD6

    别名

    CD6

    背景

    Synonyms: OX-52 Antigen, Pan-T Cells
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