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CD34 抗体 (Biotin)

Name: CD34 抗体 (Biotin)
SKU: ABIN2689204
Availability: OutOfStock

Cited in 9+ publications. The Biotin-conjugated 小鼠单克隆 anti-CD34 antibody (Clone 2E9) (ABIN2689204) specifically detects CD34 in FACS. The antibody is reactive with 犬 samples.

(9 references)

产品编号 ABIN2689204

发货至: 中国

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Quick Overview for CD34 抗体 (Biotin) (ABIN2689204)

抗原

See all CD34 抗体

CD34

适用

犬

宿主

小鼠

克隆类型

单克隆

标记

This CD34 antibody is conjugated to Biotin

应用范围

Flow Cytometry (FACS)

克隆位点

2E9

Discover our top product CD34 Primary Antibody

产品特性

The 2E9 antibody reacts with CD34, an ~110 kDa glycoprotein on the surface of bone marrow-derived progenitors of hematopoietic and endothelial cells. In the bone marrow, 1-3 % of cells are stained with 2E9 mAb, whereas peripheral blood leukocytes are not stained. Immunomagnetic depletion of lineage-committed leukocytes from bone marrow results in about three-fold enrichment of CD34+ cells. CD34+ hematopoietic progenitors may be mobilized to the peripheral blood treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell factor. Furthermore, CD34 is expressed on some canine leukemias. In the mouse, CD34 is also expressed on high endothelial venules (HEV) of lymph nodes and, in this form, functions as a ligand for L-selectin. CD34 expression on HEV of dog lymph nodes has been demonstrated with polyclonal anti-CD34 antibody.

BD Pharmingen™ Biotin Mouse Anti-Canine CD34 - Biotin - Clone 2E9 - Isotype Mouse IgG1, κ - 0.1 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

CD34-mouse IgG2a fusion protein and canine myelomonocytic leukemia ML3

亚型

IgG1 kappa
anti-CD34 (CD34) (AA 366-382), (C-Term) antibody
CD34 适用: 人, 大鼠, 小鼠 FACS, WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) (AA 201-300) antibody
CD34 适用: 人, 大鼠, 小鼠, 兔 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) (AA 301-385) antibody
CD34 适用: 人, 大鼠, 小鼠, 兔, 小鸡, 山羊 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) (AA 36-287) antibody
CD34 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) (C-Term) antibody
CD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) antibody
CD34 适用: 人, 大鼠 FACS, IHC, StM 宿主: 兔 Monoclonal HPCA1-2598R unconjugated Recombinant Antibody

anti-CD34 (CD34) antibody
CD34 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p), IHC (fro), Func 宿主: 小鼠 Monoclonal QBEnd-10 unconjugated

anti-CD34 (CD34) antibody (PE)
CD34 适用: 人 FACS, ICC 宿主: 小鼠 Monoclonal 4H11[APG] PE

anti-CD34 (CD34) (AA 151-385) antibody
CD34 适用: 人, 大鼠 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD34 (CD34) (AA 32-290) antibody
CD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

储存条件

4 °C

储存方法

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Georges, Storb, Bruno, Brodie, Thompson, Taranova, Zaucha, Little, Zellmer, Moore, Gooley, Sale, Kiem, Sandmaier, Lyons, Nash: "Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes." in: Blood, Vol. 98, Issue 12, pp. 3447-55, (2001) (PubMed).

Goerner, Horn, Peterson, Kurre, Storb, Rasko, Kiem: "Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors." in: Blood, Vol. 98, Issue 7, pp. 2065-70, (2001) (PubMed).

Niemeyer, Hudson, Bridgman, Spano, Nash, Lothrop: "Isolation and characterization of canine hematopoietic progenitor cells." in: Experimental hematology, Vol. 29, Issue 6, pp. 686-93, (2001) (PubMed).

Bhattacharya, McSweeney, Shi, Bruno, Ishida, Nash, Storb, Sauvage, Hammond, Wu: "Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells." in: Blood, Vol. 95, Issue 2, pp. 581-5, (2000) (PubMed).

Hägglund, McSweeney, Mathioudakis, Bruno, Georges, Gass, Moore, Sale, Storb, Nash: "Ex vivo expansion of canine dendritic cells from CD34+ bone marrow progenitor cells." in: Transplantation, Vol. 70, Issue 10, pp. 1437-42, (2000) (PubMed).

Bruno, Nash, Wallace, Gass, Thompson, Storb, McSweeney: "CD34+ selected bone marrow grafts are radioprotective and establish mixed chimerism in dogs given high dose total body irradiation." in: Transplantation, Vol. 68, Issue 3, pp. 338-44, (1999) (PubMed).

McSweeney, Rouleau, Wallace, Bruno, Andrews, Krizanac-Bengez, Sandmaier, Storb, Wayner, Nash: "Characterization of monoclonal antibodies that recognize canine CD34." in: Blood, Vol. 91, Issue 6, pp. 1977-86, (1998) (PubMed).

McSweeney, Rouleau, Storb, Bolles, Wallace, Beauchamp, Krizanac-Bengez, Moore, Sale, Sandmaier, de Revel, Appelbaum, Nash: "Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein." in: Blood, Vol. 88, Issue 6, pp. 1992-2003, (1996) (PubMed).

Baumheter, Singer, Henzel, Hemmerich, Renz, Rosen, Lasky: "Binding of L-selectin to the vascular sialomucin CD34." in: Science (New York, N.Y.), Vol. 262, Issue 5132, pp. 436-8, (1993) (PubMed).
抗原

CD34

别名

CD34

背景

Synonyms: gp 105-120