(4 references)-
- 抗原 See all TRAIL (TNFSF10) 抗体
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This TRAIL antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The N2B2 antibody reacts with mouse TRAIL (TNF-Related Apoptosis-Inducing Ligand, also known as APO-2 ligand or Ly-81), a 40- kDa type-II transmembrane protein of the TNF family. This antibody recognizes mouse Trail-transfected 2PK-3 cells, but not the parental cell line. TRAIL selectively induces apoptosis in various transformed cells, but not in normal cells. Five receptors have been identified for human TRAIL, whereas only one receptor has been described for the mouse counterpart thus far. The pattern of expression of mouse TRAIL is controversial. TRAIL has been detected, with a rabbit polyclonal antibody, on the surface of activated CD4+ and CD8+ T lymphocytes, as well as on CD45R/B220+ lymphocytes, and transformed T- and B-cell lines. Similar results were obtained by western blot and mRNA analyses. However, these results could not be reproduced with the N2B2 mAb. TRAIL expression, detected with the N2B2 mAb, is induced on CD3- NK1.1+ cells after stimulation with IL-2 or IL-15. However, neither freshly isolated nor activated T cells showed TRAIL expression on the cell surface. Furthermore, TRAIL mediates NK-cell cytotoxicity, which is blocked by the N2B2 mAb. TRAIL has been renamed as CD253 recently.
BD Pharmingen™ Purified Rat Anti-Mouse CD253 - Purified - Clone N2B2 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse Trail-transfected 2PK-3 cells
- 克隆位点
- N2B2
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product TNFSF10 Primary Antibody
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anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 39-250) antibody
TNFSF10 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 185-281) antibodyTNFSF10 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) antibodyTNFSF10 适用: 人 FACS, Func 宿主: 小鼠 Monoclonal 2E5 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) antibody (PE)TNFSF10 适用: 人 FACS 宿主: 小鼠 Monoclonal 2E5 PE
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 115-287) antibodyTNFSF10 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 95-281) antibodyTNFSF10 适用: 人 WB, FACS, ICC, IHC (f) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-300) antibodyTNFSF10 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) antibodyTNFSF10 适用: 人 WB, ELISA, IHC, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (AA 1-300) antibodyTNFSF10 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) (Internal Region) antibodyTNFSF10 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "To die or not to die--the quest of the TRAIL receptors." in: Journal of leukocyte biology, Vol. 65, Issue 5, pp. 535-42, (1999) (PubMed).
: "Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 4, pp. 1906-13, (1999) (PubMed).
: "Surface expression of TRAIL/Apo-2 ligand in activated mouse T and B cells." in: European journal of immunology, Vol. 28, Issue 5, pp. 1492-8, (1998) (PubMed).
: "Differential regulation of TRAIL and CD95 ligand in transformed cells of the T and B lymphocyte lineage." in: European journal of immunology, Vol. 28, Issue 3, pp. 973-82, (1998) (PubMed).
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: "To die or not to die--the quest of the TRAIL receptors." in: Journal of leukocyte biology, Vol. 65, Issue 5, pp. 535-42, (1999) (PubMed).
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- 抗原
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- 别名
- CD253 (TNFSF10 产品)
- 别名
- APO2L antibody, Apo-2L antibody, CD253 antibody, TL2 antibody, TRAIL antibody, A330042I21Rik antibody, AI448571 antibody, APO-2L antibody, Ly81 antibody, Trail antibody, tl2 antibody, apo2l antibody, cd253 antibody, trail antibody, apo-2l antibody, trail1 antibody, xtrail1 antibody, tnfsf10l2 antibody, zgc:92320 antibody, TNLG6A antibody, TNF superfamily member 10 antibody, tumor necrosis factor (ligand) superfamily, member 10 antibody, tumor necrosis factor superfamily member 10 antibody, TNF superfamily member 10 S homeolog antibody, TNFSF10 antibody, Tnfsf10 antibody, tnfsf10.S antibody, tnfsf10 antibody
- 背景
- Synonyms: TRAIL, APO-2L, Ly-81, TL2
- 途径
- Apoptosis, Positive Regulation of Endopeptidase Activity
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