(2 references)-
- 抗原 See all CCR5 抗体
- CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))
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抗原表位
- AA 9-30
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CCR5 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The C34-3448 antibody reacts with mouse chemokine receptor CD195 (also known as CCR5), a member of the β- chemokine receptor family. CD195 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals a response to at least three chemokines: RANTES and macrophage inflammatory protein-1 (MIP-1)α and β. In the mouse system, the gene for CD195 has been mapped to chromosome 9. CD195 mRNA is expressed in heart, spleen and liver tissues, and by macrophages and T-lymphocytes. The immunogen used to generate the C34- 3448 hybridoma was a KLH-conjugated peptide consisting of amino acids 9-30 of mouse CD195 (CCR5). Expression of mouse CD195 by stimulated mouse splenocytes. BALB/c splenocytes were stimulated for 24 hours with ConA (10 μg/mL, final concentration, Sigma). The splenocytes were cultured for additional 5 days in the presence of mouse IL-2 (100 U/mL, final concentration, Cat. No. 550069). The splenocytes were stained with purified rat anti-mouse CD195 antibody (C34- 3448), followed by biotinylated mouse anti-rat IgG2c (A92-1, Cat. No. 553909) and Streptavidin- hycoerythrin (Cat. No. 554061) following BD Pharmingen's staining protocol (see Figure, Left panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed using the purified rat IgG2c isotype control (purified A23-1, Cat. No. 553982, Right panel). The quadrant markers for the bivariate dot plots were set based on the autofluorescence control and isotype control.
BD Pharmingen™ Purified Rat Anti-Mouse CD195 - Purified - Clone C34-3448 - Isotype Rat IgG2c, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse CCR5 aa. 9-30
- 克隆位点
- C34
- 亚型
- IgG2c kappa
- Top Product
- Discover our top product CCR5 Primary Antibody
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anti-Chemokine (C-C Motif) Receptor 5 (CCR5) antibody
CCR5 适用: 人 IHC (fro) 宿主: 小鼠 Monoclonal 12D1 unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) antibodyCCR5 适用: 人, 大鼠 WB, ELISA, FACS, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 6G11D1 unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) (AA 19-34), (N-Term) antibodyCCR5 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) antibodyCCR5 适用: 人 WB, ELISA, FACS, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 6H3D5 unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) (AA 201-300) antibodyCCR5 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) (AA 151-200) antibodyCCR5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) antibodyCCR5 适用: 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) (AA 1-352) antibodyCCR5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) (AA 1-352) antibodyCCR5 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 5 (CCR5) (pSer336) antibodyCCR5 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor." in: The Journal of biological chemistry, Vol. 271, Issue 24, pp. 14445-51, (1996) (PubMed).
: "Identification of cell surface receptors for the Act-2 cytokine." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 285-9, (1990) (PubMed).
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: "Cloning and characterization of a novel murine macrophage inflammatory protein-1 alpha receptor." in: The Journal of biological chemistry, Vol. 271, Issue 24, pp. 14445-51, (1996) (PubMed).
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- 抗原
- CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))
- 别名
- CD195 (CCR5 产品)
- 别名
- CC-CKR-5 antibody, CCCKR5 antibody, CCR-5 antibody, CD195 antibody, CKR-5 antibody, CKR5 antibody, CMKBR5 antibody, IDDM22 antibody, Ckr5 antibody, Cmkbr5 antibody, CCR1-L antibody, GPCR antibody, CCR5 antibody, AM4-7 antibody, C-C motif chemokine receptor 5 (gene/pseudogene) antibody, chemokine (C-C motif) receptor 5 antibody, C-C motif chemokine receptor 5 antibody, CCR5 antibody, Ccr5 antibody
- 背景
- Synonyms: CCR5
- 途径
- Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size
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