IL7R 抗体 (Biotin)
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北京 101111
Quick Overview for IL7R 抗体 (Biotin) (ABIN2688948)
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See all IL7R 抗体适用
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产品特性
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Monoclonal antibody hIL-7R-M21 reacts with the 60-90 kDa glycoprotein, CD127. CD127 is also known as the IL-7 receptor alpha (IL-7Rα) subunit. The IL-7 receptor complex is a heterodimer composed of CD127 and the common gamma chain (γc, CD132), shared by other cytokine receptors (IL-2R, IL-4R, IL-9R, IL-15R, and IL-21R). CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. In vitro experiments show the expression of CD127 is down-regulated following T cell activation. Studies indicate that the IL-7 Receptor plays an important role in the proliferation and differentiation of mature T cells. Recently, it has been shown that low surface expression of CD127, in combination with intermediate to high surface expression of CD25, the α chain of the IL-2 receptor complex, can distinguish between human regulatory and conventional CD4+ T cells in human adult and cord blood, lymph nodes and thymus. Profile of CD127 (hIL-7R-M21) expression on peripheral blood lymphocytes. Human blood cells were stained with Biotin-anti CD127 (solid line) or Biotin mouse IgG1 isotype control (dashed line, Cat. No. 555747) followed by Streptavidin-PE (Cat. No. 554061). Erythrocytes were lysed using BD PharmLyse™ lysis buffer (Cat. No. 555899). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Biotin Mouse anti-Human CD127 - Biotin - Clone HIL-7R-M21 - Isotype Mouse IgG1, κ - Reactivity Hu - 100 Tests -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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亚型
- IgG1 kappa
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anti-Interleukin 7 Receptor (IL7R) (AA 173-260) antibody
IL7R 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (AA 21-239) antibodyIL7R 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3F5D9 unconjugated
anti-Interleukin 7 Receptor (IL7R) (AA 21-239) antibodyIL7R 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (N-Term) antibodyIL7R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (AA 21-236) antibodyIL7R 适用: 人 WB, ELISA, Coat 宿主: 小鼠 Monoclonal IL7R-2751 unconjugated
anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibodyIL7R 适用: 人 FACS, WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) antibodyIL7R 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) antibody (APC)IL7R 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal A7R34 APC
anti-Interleukin 7 Receptor (IL7R) (Ser552) antibodyIL7R 适用: 人, 小鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (AA 1-459) antibodyIL7R 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 20 μL
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限制
- 仅限研究用
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells." in: The Journal of experimental medicine, Vol. 203, Issue 7, pp. 1693-700, (2006) (PubMed).
: "CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells." in: The Journal of experimental medicine, Vol. 203, Issue 7, pp. 1701-11, (2006) (PubMed).
: "Interleukin-7 is a critical growth factor in early human T-cell development." in: Blood, Vol. 88, Issue 11, pp. 4239-45, (1996) (PubMed).
: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).
: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." in: Cell, Vol. 60, Issue 6, pp. 941-51, (1990) (PubMed).
: "Stimulation of B-cell progenitors by cloned murine interleukin-7." in: Nature, Vol. 333, Issue 6173, pp. 571-3, (1988) (PubMed).
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: "Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells." in: The Journal of experimental medicine, Vol. 203, Issue 7, pp. 1693-700, (2006) (PubMed).
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- IL7R (Interleukin 7 Receptor (IL7R))
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别名
- CD127
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背景
- Synonyms: IL-7R, IL7R, IL7RA, IL-7Rα, IL-7R-alpha, Interleukin-7 Receptor alpha
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
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