CD11b 抗体
(8 references)-
- 抗原 See all CD11b (ITGAM) 抗体
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
-
适用
- 人, 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This CD11b antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 产品特性
-
The M1/70 antibody reacts with the 170 kDa α[M] chain of Mac-1 (CD11b/CD18, α[M]β2 integrin), also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 cells. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. M1/70 antibody reportedly blocks cell adherence and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of CD11b on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified Rat IgG2b, κ isotype control A95-1 (Cat. No. 553986, left panel) or purified M1/70 monoclonal antibodies (right panel), followed by FITC-conjugated goat anti-rat Ig (Cat. No. 554016, both panels). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Purified Rat Anti-Mouse CD11b - Purified - Clone M1/70 - Isotype Rat IgG2b, κ - Reactivity Ms, Hu - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- C57BL/10 splenic T cells and concanavalin A-activated C57BL/10 splenocytes
- 克隆位点
- M1-70
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product ITGAM Primary Antibody
-
anti-Integrin alpha M (ITGAM) (AA 520-570) antibody
ITGAM 适用: 人, 小鼠, 大鼠 FACS 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 145-340) antibodyITGAM 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 77-180) antibodyITGAM 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (fro), ELISA, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 253-282), (N-Term) antibodyITGAM 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB17991 unconjugated
anti-Integrin alpha M (ITGAM) (AA 31-160) antibodyITGAM 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 941-1074) antibodyITGAM 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal ITGAM-3338 unconjugated
anti-Integrin alpha M (ITGAM) (AA 941-1074) antibodyITGAM 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal ITGAM-3340 unconjugated
anti-Integrin alpha M (ITGAM) antibody (Biotin)ITGAM 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-174 Biotin
anti-Integrin alpha M (ITGAM) antibody (FITC)ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 FITC
anti-Integrin alpha M (ITGAM) antibody (PE)ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 PE
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
-
-
: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).
: "Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54)." in: Journal of leukocyte biology, Vol. 59, Issue 5, pp. 648-55, (1996) (PubMed).
: "Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors." in: Science (New York, N.Y.), Vol. 245, Issue 4923, pp. 1238-41, (1989) (PubMed).
: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, (1983) (PubMed).
: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, (1983) (PubMed).
: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, (1983) (PubMed).
: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, (1979) (PubMed).
: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, (1978) (PubMed).
-
: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).
-
- 抗原
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
- 别名
- CD11b (ITGAM 产品)
- 别名
- CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody
- 背景
- Synonyms: Integrin α[M] chain, Mac-1 α chain, CR3
- 途径
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
-
