(3 references)-
- 抗原 See all Caspase 3 (CASP3) 抗体
- Caspase 3 (CASP3)
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This Caspase 3 antibody is conjugated to Biotin
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应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Pharmingen™
- 产品特性
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The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the pro-enzyme form of caspase-3. Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells (Human T-cell leukemia, ATCC TIB-152) were left untreated (left panel) or treated with 4 μM of camptothecin for 4 hr to induce apoptosis (right panel). Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714) for 20 min at room temperature (RT), pelleted and washed with BD Perm/Wash™ buffer (component of Cat. No. 554714). Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody (clone C92-605). Cells were then washed and resuspended in BD Perm/Wash™ buffer containing FITC-Avidin (5 ng/1x10e6 cells) [Cat. No. 554057] and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash™ buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 (left panel, M1), whereas more than half of the treated cells were positive for active caspase-3 staining (right panel, M2).
BD Pharmingen™ Biotin Rabbit Anti- Active Caspase-3 - Biotin - Clone C92-605 - Isotype Rabbit IgG - Reactivity Hu, Ms - 100 Tests - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 克隆位点
- C92
- 亚型
- IgG
- Top Product
- Discover our top product CASP3 Primary Antibody
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anti-Caspase 3 (CASP3) (AA 60-100) antibody
CASP3 适用: 人, 小鼠, 大鼠, Cow, 山羊 WB, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 67-175) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 55-160) antibodyCASP3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 220-236), (C-Term) antibodyCASP3 适用: 人 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 11-120) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal 5E1 unconjugated
anti-Caspase 3 (CASP3) (cleaved) antibodyCASP3 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (Internal Region) antibodyCASP3 适用: 人, 小鼠, 大鼠, Cow WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (pSer150) antibodyCASP3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 (CASP3) (AA 100-200) antibodyCASP3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 20 μL
- 限制
- 仅限研究用
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- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells." in: Blood, Vol. 93, Issue 10, pp. 3309-16, (1999) (PubMed).
: "Involvement of Bcl-2 cleavage in the acceleration of VP-16-induced U937 cell apoptosis." in: Biochemical and biophysical research communications, Vol. 246, Issue 2, pp. 484-8, (1998) (PubMed).
: "Caspases: enemies within." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1312-6, (1998) (PubMed).
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: "Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells." in: Blood, Vol. 93, Issue 10, pp. 3309-16, (1999) (PubMed).
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- 抗原
- Caspase 3 (CASP3)
- 别名
- Caspase-3 (CASP3 产品)
- 别名
- CPP32 antibody, CPP32B antibody, SCA-1 antibody, A830040C14Rik antibody, AC-3 antibody, Apopain antibody, CC3 antibody, Caspase-3 antibody, Lice antibody, Yama antibody, mldy antibody, xcpp32 antibody, casp3 antibody, zgc:100890 antibody, CASP-3 antibody, caspase-3 antibody, caspase 3 antibody, caspase 3 S homeolog antibody, caspase 3, apoptosis-related cysteine peptidase a antibody, caspase 3, apoptosis-related cysteine peptidase antibody, CASP3 antibody, Casp3 antibody, casp3.S antibody, casp3a antibody
- 背景
- Synonyms: CPP32, Yama, Apopain
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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