BrdU 抗体
Quick Overview for BrdU 抗体 (ABIN2688858)
抗原
See all BrdU 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the vells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics. The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU. Fluorescence Intensity (Linear) Profile of KG1A cells analyzed ona FACScan
BD Pharmingen™ Purified Mouse Anti- BrdU - Purified - Clone 3D4 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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亚型
- IgG1 kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain." in: The Journal of comparative neurology, Vol. 521, Issue 3, pp. 677-96, (2013) (PubMed).
: "Ontogeny of cone photoreceptor mosaics in zebrafish." in: The Journal of comparative neurology, Vol. 518, Issue 20, pp. 4182-95, (2011) (PubMed).
: "Antidepressant drug-induced stimulation of mouse hippocampal neurogenesis is age-dependent and altered by early life stress." in: The Journal of comparative neurology, Vol. 509, Issue 4, pp. 372-81, (2008) (PubMed).
: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." in: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).
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: "Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain." in: The Journal of comparative neurology, Vol. 521, Issue 3, pp. 677-96, (2013) (PubMed).
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- BrdU (Bromodeoxyuridine (BrdU))
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别名
- BrdU
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物质类
- Chemical
抗原
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