Leukotriene B4 Receptor/BLT 抗体 (Biotin)
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Quick Overview for Leukotriene B4 Receptor/BLT 抗体 (Biotin) (ABIN2688854)
抗原
See all Leukotriene B4 Receptor/BLT (LTB4R) 抗体适用
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克隆类型
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应用范围
克隆位点
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产品特性
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The monoclonal antibody 203/14F11 reacts against human leukotriene B4 receptor 1 (BLTR-1). BLTR-1 is a seven-transmembrane, G-protein-coupled receptor. The receptor plays an important role in pro-inflammatory responses. Leukotrienne B4 receptors are distributed in various hematopoietic cells including monocytes, granulocytes, and lymphocytes. Leukotriene B4 (LTB4), a product of arachidonic acid metabolism, is the ligand for BLTR-1. The immunogen used to generate 203/14F11 hybridoma was a human BLTR-1-transfected cell line. Detection of BLTR-1 expression on human peripheral monocytes and granulocytes by biotinylated 203/14F11. Human lysed whole blood were stained with 20 μL/test of biotinylated 203/14F11. Streptavidin-PE (Cat. No. 554061) was used as second-step reagent. The data reflects gating on monocytes (left panel) and granulocytes (right panel) based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated mouse IgG1 (Cat. No. 555747) as isotype control (shaded histogram).
BD Pharmingen™ Biotin Mouse Anti-Human BLTR-1(Leukotriene B4 Receptor) - Biotin - Clone 203/14F11 - Isotype Mouse IgG1 - Reactivity Hu - 100 Tests -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human BLTR-1-transfected cell line
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亚型
- IgG1
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anti-Leukotriene B4 Receptor (LTB4R) (AA 101-200) antibody
LTB4R 适用: 人, 小鼠, 大鼠 FACS, IHC (fro), IHC (p), ELISA, IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) (Cytoplasmic Domain) antibodyLTB4R 适用: 人, 小鼠, 大鼠, 猴, Hamster, 马, 兔 IHC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) (C-Term) antibodyLTB4R 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) antibodyLTB4R 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) (AA 168-181), (Extracellular) antibodyLTB4R 适用: 人 FACS, WB 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) antibodyLTB4R 适用: 人 WB 宿主: 兔 Monoclonal 24GB50 unconjugated Recombinant Antibody
anti-Leukotriene B4 Receptor (LTB4R) antibodyLTB4R 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Leukotriene B4 Receptor (LTB4R) (AA 250-299) antibodyLTB4R 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 20 μL
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限制
- 仅限研究用
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 520-5, (2001) (PubMed).
: "Distribution of leukotriene B4 receptors in human hematopoietic cells." in: Immunopharmacology, Vol. 48, Issue 2, pp. 157-63, (2000) (PubMed).
: "Leukotriene B4 receptor. Cloning and intracellular signaling." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 2 Pt 2, pp. S51-5, (2000) (PubMed).
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: "First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 520-5, (2001) (PubMed).
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- Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))
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别名
- BLTR-1Leukotriene B4 Receptor
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背景
- Synonyms: hBLTR-1
抗原
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