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PRDM1 抗体

Name: PRDM1 抗体
SKU: ABIN2688853
Availability: OutOfStock

Cited in 8+ publications. The 大鼠单克隆 anti-PRDM1 antibody (Clone 6D3) (ABIN2688853) specifically detects PRDM1 in WB, IHC (p) 和 ICS. The antibody is reactive with 人和小鼠 samples.

(8 references)

产品编号 ABIN2688853

发货至: 中国

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Quick Overview for PRDM1 抗体 (ABIN2688853)

抗原

See all PRDM1 抗体

PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

适用

人, 小鼠

宿主

大鼠

克隆类型

单克隆

标记

This PRDM1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Intracellular Staining (ICS)

克隆位点

6D3

Discover our top product PRDM1 Primary Antibody

产品特性

The 6D3 monoclonal antibody specifically binds to mouse B lymphocyte-induced maturation protein 1 (Blimp-1) and crossreacts with human BLIMP-1. Blimp-1 is a 98 kDa zinc finger-containing protein that is encoded by the Prdm1 gene. Blimp-1 was first discovered as a transcriptional repressor of the IFN-β promoter. Blimp-1 is critical for primordial germ cells specification in the early embryo and induction of terminal cell differentiation in multiple somatic cell types. It is largely known as a master regulator of antibody-secreting plasma cells due to its function in the repression of PAX5, CIITA and cMYC genes. Blimp-1 also controls T cell homeostasis and effector differentiation through different mechanisms, including repression of IL-17 producing cells. Blimp-1 also plays roles in the maturation and activity of dendritic cells and natural killer cells. Analyses of Blimp-1 Expression Panel 1. Western blot analysis of Blimp-1 expression in human myeloma cell lines. Lysates from RPMI-8226 (ATCC CLL-155, Lane 1) and U266 (ATCC TIB-196, Lane 2) cells were electrophoresed, blotted and probed with Purified Rat Anti-Blimp-1 antibody (Cat. No. 564703) followed by HRP Goat Anti-Rat Ig (Cat. No. 554017), and ECL detection reagents. The antibody detects Blimp-1 isoforms of 88-92 kDa in both cell lysates and 76-78 kDa) in the U266 cell lysate. Note: an unspecific band (~250 kDa) may be observed upon overexposure of the membranes. Panel 2. Flow cytometric analysis of Blimp-1 expression in a human myeloma cell line. RPMI 8226 cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725) and stained with either Purified Rat IgG2a, κ (Cat. No. 553927, dashed line histogram) or Purified Rat Anti-Blimp-1 antibody (solid line histogram) followed by staining with PE Goat Anti-Rat Ig (Cat. No. 560767). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Panel 3. Immunohistochemical staining of human Blimp-1 in human tonsils. Following antigen retrieval with BD Retrievagen A Buffer (Cat No. 550524), the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Rat IgG2a κ Isotype Control (Left Image) or Purified Rat Anti-Human Blimp-1 antibody (Right Image). A three-step staining procedure that employs Biotin Goat Anti-Rat Immunoglobulin (Cat. No. 559286), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. As shown in the Right Image, the Blimp-1 specific antibody primarily stained the nuclei of some Germinal Center cells. Original magnification: 40x.

BD Pharmingen™ Purified Rat Anti-Blimp-1 - Purified - Clone 6D3 - Isotype Rat IgG2a, κ - Reactivity Hu, Ms - 0.1 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse Blimp-1 Recombinant Protein

亚型

IgG2a kappa
anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 1-109) antibody
PRDM1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2B10 unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (Internal Region) antibody
Verified PRDM1 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 690-825) antibody
PRDM1 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2F1B6 unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 1-109) antibody
PRDM1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F8 unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 422-493) antibody
PRDM1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G9 unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 690-825) antibody
PRDM1 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 7C11H1 unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (C-Term) antibody
Verified PRDM1 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (Middle Region) antibody
PRDM1 适用: 人, 小鼠, 大鼠, 犬, Pig, Cow, 马, 兔, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 199-409) antibody
PRDM1 适用: 人, 小鼠, Pig WB, ELISA, IHC, IP, ICC, FACS, IF 宿主: 小鼠 Monoclonal 3H2-E8 unconjugated

anti-PR Domain Containing 1, with ZNF Domain (PRDM1) (AA 29-149) antibody
PRDM1 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Chan, Chiang, Tsai, Su, Chen, Hou, Lin: "Absence of the transcriptional repressor Blimp-1 in hematopoietic lineages reveals its role in dendritic cell homeostatic development and function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7039-46, (2009) (PubMed).

Johnston, Poholek, DiToro, Yusuf, Eto, Barnett, Dent, Craft, Crotty: "Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation." in: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1006-10, (2009) (PubMed).

Jourdan, Caraux, De Vos, Fiol, Larroque, Cognot, Bret, Duperray, Hose, Klein: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).

Martins, Cimmino, Liao, Magnusdottir, Calame: "Blimp-1 directly represses Il2 and the Il2 activator Fos, attenuating T cell proliferation and survival." in: The Journal of experimental medicine, Vol. 205, Issue 9, pp. 1959-65, (2008) (PubMed).

Ohinata, Payer, OCarroll, Ancelin, Ono, Sano, Barton, Obukhanych, Nussenzweig, Tarakhovsky, Saitou, Surani: "Blimp1 is a critical determinant of the germ cell lineage in mice." in: Nature, Vol. 436, Issue 7048, pp. 207-13, (2005) (PubMed).

Shaffer, Lin, Kuo, Yu, Hurt, Rosenwald, Giltnane, Yang, Zhao, Calame, Staudt: "Blimp-1 orchestrates plasma cell differentiation by extinguishing the mature B cell gene expression program." in: Immunity, Vol. 17, Issue 1, pp. 51-62, (2002) (PubMed).

Turner, Mack, Davis: "Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells." in: Cell, Vol. 77, Issue 2, pp. 297-306, (1994) (PubMed).

Keller, Maniatis: "Identification and characterization of a novel repressor of beta-interferon gene expression." in: Genes & development, Vol. 5, Issue 5, pp. 868-79, (1991) (PubMed).
抗原

PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

别名

Blimp-1

背景

Synonyms: BLIMP-1, PRDM1, PR domain containing 1, with ZNF domain, PRDI-BF1

途径

Regulation of Muscle Cell Differentiation