TNFSF9 抗体
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Quick Overview for TNFSF9 抗体 (ABIN2688839)
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See all TNFSF9 抗体适用
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产品特性
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The TKS-1 antibody reacts with 4-1BB Ligand (4-1BBL), a 97 kDa member of the NGF/TNF superfamily which is present on antigen-presenting cells and activated B lymphocytes. 4-1BBL interacts with 4-1BB (CDw137), found predominantly on activated T lymphocytes. This ligand-receptor pair is grouped with pairs such as CD27/CD70, CD30/CD153, OX-40/OX-40L, CD80/CD28, and CD86/CD28, which costimulate T-cell proliferation and cytokine production. The interaction of 4-1BB with 4-1BBL may be reciprocally costimulatory in that 4-1BBL may deliver proliferative signals to B lymphocytes activated by anti-IgM. The TKS-1 mAb stains B cells activated for three days with anti-IgM polyclonal antibody, consistent with an earlier report that soluble 4-1BB-alkaline phosphatase fusion protein binds to anti-IgM-activated B cells. Furthermore, 4-1BBL mRNA has been detected in activated T lymphocytes and activated macrophages. The TKS-1 mAb has been reported to inhibit the binding of soluble 4-1BB to 4-1BBL transfectants and block the in vitro costimulation of T-lymphocyte proliferation by 4-1BBL transfectants. Expression of 4-1BB Ligand (4-1BBL) on mouse splenocytes. C57BL/6 splenocytes, freshly isolated (left two panels) and following 3-day activation with soluble anti-mouse IgM (Jackson Immunoresearch, right two panels), were stained with purified rat IgG2a, κ isotype control mAb R35-95 (Cat. no. 553927, far left and center right panels) or purified mAb TKS-1 (center left and far right panels), followed by PE-conjugated goat anti-rat Ig (Cat. no. 550767, all panels), then FITC-conjugated anti-mouse CD45R/B220 mAb RA3-6B2 (Cat. no. 553087/553088, all panels). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Rat Anti-Mouse 4-1BB Ligand - Purified - Clone TKS-1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- BALB/c mouse B lymphoma line 2PK-3
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亚型
- IgG2a kappa
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anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 101-200) antibody
TNFSF9 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 42-253) antibodyTNFSF9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 105-309) antibodyTNFSF9 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) antibodyTNFSF9 适用: 人 FACS, IHC, StM 宿主: 小鼠 Monoclonal CD137L-1547 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 52-254) antibodyTNFSF9 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM68 unconjugated Recombinant Antibody
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (Extracellular Domain) antibodyTNFSF9 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 41B436 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 52-254) antibodyTNFSF9 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) antibodyTNFSF9 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB2375 unconjugated Recombinant Antibody
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 145-254) antibodyTNFSF9 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) antibodyTNFSF9 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis." in: The Journal of experimental medicine, Vol. 191, Issue 2, pp. 375-80, (2000) (PubMed).
: "Costimulation of CD28- T lymphocytes by 4-1BB ligand." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 551-9, (1997) (PubMed).
: "Regulation of 4-1BB expression by cell-cell interactions and the cytokines, interleukin-2 and interleukin-4." in: European journal of immunology, Vol. 25, Issue 2, pp. 488-94, (1995) (PubMed).
: "Molecular cloning of a ligand for the inducible T cell gene 4-1BB: a member of an emerging family of cytokines with homology to tumor necrosis factor." in: European journal of immunology, Vol. 23, Issue 10, pp. 2631-41, (1993) (PubMed).
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: "Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis." in: The Journal of experimental medicine, Vol. 191, Issue 2, pp. 375-80, (2000) (PubMed).
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- TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
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别名
- 4-1BB Ligand
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途径
- Activated T Cell Proliferation, Cancer Immune Checkpoints
抗原
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