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14-3-3 theta 抗体

The 小鼠 单克隆 anti-14-3-3 theta antibody is suitable to detect 14-3-3 theta in samples from 人, 小鼠, 大鼠, 小鸡, 犬 和 Frog. It has been validated for WB, IHC, IF 和 IP.
产品编号 ABIN2688837
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for 14-3-3 theta 抗体 (ABIN2688837)

抗原

See all 14-3-3 theta (YWHAQ) 抗体
14-3-3 theta (YWHAQ)

适用

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人, 小鼠, 大鼠, 小鸡, 犬, Frog

宿主

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小鼠

克隆类型

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单克隆

标记

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This 14-3-3 theta antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

12-14-3
  • 产品特性

    The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling. The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase. It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway. However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity. It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ. Western blot analysis of 14-3-3 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2.2). Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Mouse Anti- 14-3-3 antibody. Immunofluorescence staining of SKN cells (Human neuroblastoma).

    BD Transduction Laboratories™ Purified Mouse Anti- 14-3-3 - Purified - Clone 12/14-3-3 - Isotype Mouse IgM - Reactivity Hu, Chick, Dog, Frog, Ms, Rat - 50 μg

    纯化方法

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    免疫原

    Human 14-3-3

    亚型

    IgM
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    250 μg/mL

    缓冲液

    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store undiluted at -20°C.
  • 抗原

    14-3-3 theta (YWHAQ)

    别名

    14-3-3

    分子量

    29 kDa

    途径

    Apoptosis, Myometrial Relaxation and Contraction
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