14-3-3 theta 抗体
Quick Overview for 14-3-3 theta 抗体 (ABIN2688837)
抗原
See all 14-3-3 theta (YWHAQ) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling. The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase. It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway. However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity. It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ. Western blot analysis of 14-3-3 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2.2). Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Mouse Anti- 14-3-3 antibody. Immunofluorescence staining of SKN cells (Human neuroblastoma).
BD Transduction Laboratories™ Purified Mouse Anti- 14-3-3 - Purified - Clone 12/14-3-3 - Isotype Mouse IgM - Reactivity Hu, Chick, Dog, Frog, Ms, Rat - 50 μg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human 14-3-3
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亚型
- IgM
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anti-14-3-3 theta (YWHAQ) (AA 1-245) antibody
YWHAQ 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 theta (YWHAQ) (AA 1-245) antibodyYWHAQ 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 小鼠 Monoclonal 5C4E7 unconjugated
anti-14-3-3 theta (YWHAQ) (Center) antibodyYWHAQ 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 theta (YWHAQ) antibodyYWHAQ 适用: 人, 小鼠, 大鼠 WB, IF (cc) 宿主: 兔 Monoclonal 5G1 unconjugated
anti-14-3-3 theta (YWHAQ) (C-Term) antibodyYWHAQ 适用: 人, 小鼠, 大鼠, Cow, 小鸡, 犬, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 theta (YWHAQ) antibodyYWHAQ 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-14-3-3 theta (YWHAQ) (N-Term) antibodyYWHAQ 适用: 人, 小鼠, 大鼠, Cow, 小鸡, 犬, 兔, 非洲爪蟾, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 theta (YWHAQ) (C-Term) antibodyYWHAQ 适用: 人, 小鼠, 大鼠, Cow, 小鸡, 兔, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 theta (YWHAQ) (Center) antibodyYWHAQ 适用: 人, 小鼠, 大鼠, Cow, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 theta (YWHAQ) (N-Term) antibodyYWHAQ 适用: 人, 小鼠, 大鼠, Cow, 犬, 斑马鱼, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20°C.
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- 14-3-3 theta (YWHAQ)
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别名
- 14-3-3
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分子量
- 29 kDa
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途径
- Apoptosis, Myometrial Relaxation and Contraction
抗原
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