Histone H4 抗体
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北京 101111
Quick Overview for Histone H4 抗体 (ABIN2668873)
抗原
See all Histone H4 (HISTH4) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Histone H4 antibody (pAb)
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纯化方法
- Affinity Purified
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免疫原
- This Histone H4 antibody was raised against a peptide corresponding to amino acids 43- 54 of human histone H4.
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亚型
- IgG
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应用备注
- ChIP: 2 - 10 µL per ChIP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H4, we also offer AbFlex Histone H4 Recombinant Antibody (rAb). For details, see Catalog No. 91295.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Lactate Stimulates IL-4 and IL-13 Production in Activated HuT-78 T Lymphocytes Through a Process That Involves Monocarboxylate Transporters and Protein Hyperacetylation." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 36, Issue 5, pp. 317-27, (2016) (PubMed).
: "L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation." in: Cell communication and signaling : CCS, Vol. 13, pp. 36, (2015) (PubMed).
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- Histone H4 (HISTH4)
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别名
- Histone H4
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背景
- Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression.
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分子量
- 8 kDa
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基因ID
- 8363
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NCBI登录号
- NP_778224
抗原
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