Histone H2B 抗体 (H2BK120ub)
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北京 101111
Quick Overview for Histone H2B 抗体 (H2BK120ub) (ABIN2668551)
抗原
See all Histone H2B 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- H2BK120ub
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原理
- Histone H2BK120ub1 antibody (mAb) (Clone 56)
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纯化方法
- Protein G Chromatography
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免疫原
- This Histone H2B ubiquityl Lys120 antibody was raised against a branched peptide corresponding the the ubiquitin conjugation site at Lys120 of human Histone H2B.
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亚型
- IgG2a
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anti-Histone H2B (acLys12) antibody
适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK20ac) antibody适用: 人 WB, ELISA, IF, ICC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK5ac) antibody适用: 人 WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (AA 1-126) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B antibody适用: 人, 小鼠, 大鼠 WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Histone H2B (H2BK120ac) antibody适用: 人 WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B antibody适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 3A6 unconjugated
anti-Histone H2B (H2BK15ac) antibody适用: 人 WB, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK116ac) antibody适用: 人 WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK11ac) antibody适用: 人 WB, ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Histone H2B ubiquitylation represses gametogenesis by opposing RSC-dependent chromatin remodeling at the ste11 master regulator locus." in: eLife, Vol. 5, (2016) (PubMed).
: "The PAF complex and Prf1/Rtf1 delineate distinct Cdk9-dependent pathways regulating transcription elongation in fission yeast." in: PLoS genetics, Vol. 9, Issue 12, pp. e1004029, (2014) (PubMed).
: "Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast." in: Molecular and cellular biology, Vol. 33, Issue 24, pp. 4779-92, (2013) (PubMed).
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- Histone H2B
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背景
- Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2B is ubiquitylated at lysine 120 in vertebrates. H2B ubiquityl Lys120 is found preferentially associated with the transcribed regions of highly expressed genes. It is possible that H2B ubiquitylation is associated with transcriptional elongation.
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分子量
- 26 kDa
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基因ID
- 8348
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NCBI登录号
- NP_003512
抗原
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