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Rpb1 CTD 抗体 (Thr4)

There are 4+ publications for this product available. The 大鼠 单克隆 anti-Rpb1 CTD antibody is suitable to detect Rpb1 CTD in samples from 人. It has been validated for WB, IF, ICC 和 ChIP.
产品编号 ABIN2668543
发货至: 中国
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Quick Overview for Rpb1 CTD 抗体 (Thr4) (ABIN2668543)

抗原

Rpb1 CTD (RNA Polymerase II CTD)

适用

  • 23
  • 6
  • 2
  • 2
  • 2
  • 1
  • 1

宿主

  • 10
  • 9
  • 4
大鼠

克隆类型

  • 14
  • 9
单克隆

标记

  • 23
This Rpb1 CTD antibody is un-conjugated

应用范围

  • 23
  • 17
  • 14
  • 9
  • 7
  • 4
  • 4
  • 3
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

克隆位点

6D7
  • 抗原表位

    • 8
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Thr4

    纯化方法

    Protein G Chromatography

    免疫原

    This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.

    亚型

    IgG2b
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 浓度

    1 μg/μL

    缓冲液

    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    储存条件

    -20 °C

    储存方法

    Antibodies in solution can be stored at -20 °C for 2 years.

    有效期

    6 months
  • Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

    Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

    Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

    Mayer, Heidemann, Lidschreiber, Schreieck, Sun, Hintermair, Kremmer, Eick, Cramer: "CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II." in: Science (New York, N.Y.), Vol. 336, Issue 6089, pp. 1723-5, (2012) (PubMed).

  • 抗原

    Rpb1 CTD (RNA Polymerase II CTD)

    别名

    RNA Pol II CTD

    分子量

    240 kDa

    基因ID

    5430
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