POLR2A/RPB1 抗体
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北京 101111
Quick Overview for POLR2A/RPB1 抗体 (ABIN2668535)
抗原
See all POLR2A/RPB1 (POLR2A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- RNA pol II antibody (mAb) (Clone 1F4B6)
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a synthetic peptide containing the CTD of human RNA Polymerase II.
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亚型
- IgG2a
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anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibody
POLR2A 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibodyPOLR2A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 10-321) antibodyPOLR2A 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ELISA, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB, IF, ELISA, ChIP, FM, CUT&RUN 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IF, ELISA, ChIP, FM, CUT&RUN 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 128-258) antibodyPOLR2A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1B1D8 unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Monoclonal 2G1 unconjugated Recombinant Antibody
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal CTD4H8 unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal CTD 8A7 unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Monoclonal 2H4 unconjugated Recombinant Antibody
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应用备注
- ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 5 μg/mL dilution WB*: 0.5 - 2 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. The addition of 0.1 % Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. For RNA Pol II, we also offer AbFlex RNA Pol II Recombinant Antibody (rAb). For details, see Catalog No. 91151.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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别名
- POLR2A / RNA Polymerase II
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背景
- RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
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分子量
- 240 kDa
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基因ID
- 5430
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NCBI登录号
- NP_000928
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途径
- Regulatory RNA Pathways
抗原
-
