Histone H2B 抗体 (AA 111-125)
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北京 101111
Quick Overview for Histone H2B 抗体 (AA 111-125) (ABIN2668332)
抗原
See all Histone H2B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 111-125
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原理
- Histone H2B antibody (pAb)
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纯化方法
- Protein G Chromatography
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免疫原
- This Histone H2B antibody was raised against a peptide corresponding to amino acid residues 111-125 of human Histone H2B.
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亚型
- IgG
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anti-Histone H2B (acLys12) antibody
适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK20ac) antibody适用: 人 WB, ELISA, IF, ICC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK5ac) antibody适用: 人 WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (AA 1-126) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B antibody适用: 人, 小鼠, 大鼠 WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Histone H2B (H2BK120ac) antibody适用: 人 WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B antibody适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 3A6 unconjugated
anti-Histone H2B (H2BK15ac) antibody适用: 人 WB, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK116ac) antibody适用: 人 WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK11ac) antibody适用: 人 WB, ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 2 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
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限制
- 仅限研究用
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浓度
- 0.5 μg/μL
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缓冲液
- PBS containing 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- 4 °C,-20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination." in: Molecular and cellular biology, Vol. 30, Issue 13, pp. 3216-32, (2010) (PubMed).
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- Histone H2B
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背景
- Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.
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分子量
- 15 kDa
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基因ID
- 8348
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NCBI登录号
- NP_778225
抗原
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