POLR2B 抗体 (N-Term)
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北京 101111
Quick Overview for POLR2B 抗体 (N-Term) (ABIN2668294)
抗原
See all POLR2B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- POLR2B antibody (pAb)
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纯化方法
- Affinity Purified
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免疫原
- This antibody was raised against a peptide within the N-terminal region of human POLR2B.
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亚型
- IgG
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (AA 1-260) antibody
POLR2B 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibodyPOLR2B 适用: 人, 小鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) antibodyPOLR2B 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (AA 805-854) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Pig, 非洲爪蟾, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibodyPOLR2B 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (Middle Region) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig, 非洲爪蟾, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, 非洲爪蟾, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (AA 119-168) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, 斑马鱼, 豚鼠, 马, 兔, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- IP: 5 - 10 µL per IP WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
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别名
- POLR2B
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背景
- POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa) DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
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分子量
- 135 kDa
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基因ID
- 5431
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NCBI登录号
- NP_000929
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途径
- Regulatory RNA Pathways, DNA Damage Repair
抗原
-
