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KDM4A 抗体 (AA 586-674)

The 小鼠 单克隆 anti-KDM4A antibody (Clone N154-32) (ABIN2668289) specifically detects KDM4A in WB. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN2668289
发货至: 中国
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Quick Overview for KDM4A 抗体 (AA 586-674) (ABIN2668289)

抗原

See all KDM4A 抗体
KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

适用

  • 83
  • 37
  • 27
  • 1
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  • 1
人, 小鼠

宿主

  • 69
  • 15
  • 1
小鼠

克隆类型

  • 54
  • 31
单克隆

标记

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  • 2
  • 2
  • 2
  • 2
  • 2
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  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
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This KDM4A antibody is un-conjugated

应用范围

  • 45
  • 27
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  • 13
  • 12
  • 8
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  • 7
  • 2
  • 2
  • 1
  • 1
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Western Blotting (WB)

克隆位点

N154-32
  • 抗原表位

    • 13
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
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    AA 586-674

    原理

    JMJD2A antibody (mAb) (Clone N154/32)

    纯化方法

    Protein A Chromatography

    免疫原

    This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.

    亚型

    IgG1
  • 应用备注

    WB*: 1 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91143.

    限制

    仅限研究用
  • 浓度

    1 μg/μL

    缓冲液

    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

    储存条件

    -20 °C

    储存方法

    Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

    有效期

    24 months
  • 抗原

    KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

    别名

    JMJD2A

    背景

    JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

    分子量

    130 kDa

    基因ID

    9682

    NCBI登录号

    NP_055478

    途径

    Warburg Effect
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