KDM4A 抗体 (AA 586-674)
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Quick Overview for KDM4A 抗体 (AA 586-674) (ABIN2668289)
抗原
See all KDM4A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 586-674
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原理
- JMJD2A antibody (mAb) (Clone N154/32)
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纯化方法
- Protein A Chromatography
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免疫原
- This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.
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亚型
- IgG1
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应用备注
- WB*: 1 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91143.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))
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别名
- JMJD2A
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背景
- JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.
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分子量
- 130 kDa
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基因ID
- 9682
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NCBI登录号
- NP_055478
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途径
- Warburg Effect
抗原
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