Nucleolin 抗体
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北京 101111
Quick Overview for Nucleolin 抗体 (ABIN2668241)
抗原
See all Nucleolin (NCL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Nucleolin antibody (mAb) (Clone 3G4B20)
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纯化方法
- Culture Supernatant
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免疫原
- This Nucleolin antibody was raised against a salt-extracted nuclear matrix preparation from MDCK cells.
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亚型
- IgG1
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应用备注
- ICC/IF: 1:500 dilution WB*: 1:500 - 1:2,000 dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91231. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Concentrated culture supernatant containing 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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- Nucleolin (NCL)
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别名
- Nucleolin
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背景
- Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.
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分子量
- 100 kDa
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基因ID
- 4691
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NCBI登录号
- XP_850477
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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