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Nucleolin 抗体

The 小鼠 单克隆 anti-Nucleolin antibody (Clone 3G4B20) (ABIN2668241) specifically detects Nucleolin in WB, IF 和 ICC. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN2668241
发货至: 中国
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Quick Overview for Nucleolin 抗体 (ABIN2668241)

抗原

See all Nucleolin (NCL) 抗体
Nucleolin (NCL)

适用

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人, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This Nucleolin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

3G4B20
  • 原理

    Nucleolin antibody (mAb) (Clone 3G4B20)

    纯化方法

    Culture Supernatant

    免疫原

    This Nucleolin antibody was raised against a salt-extracted nuclear matrix preparation from MDCK cells.

    亚型

    IgG1
  • 应用备注

    ICC/IF: 1:500 dilution WB*: 1:500 - 1:2,000 dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91231. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Concentrated culture supernatant containing 30 % glycerol and 0.035 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

    储存条件

    -20 °C

    储存方法

    Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

    有效期

    24 months
  • 抗原

    Nucleolin (NCL)

    别名

    Nucleolin

    背景

    Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.

    分子量

    100 kDa

    基因ID

    4691

    NCBI登录号

    XP_850477

    途径

    Ribonucleoprotein Complex Subunit Organization
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