NOS2 抗体
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北京 101111
Quick Overview for NOS2 抗体 (ABIN265479)
抗原
See all NOS2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of NOS2/iNOS protein. (region surrounding Val1131)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 14-100) antibody
NOS2 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) antibodyNOS2 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 4E5 unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 518-694) antibodyNOS2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 43-213) antibodyNOS2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 830-860) antibodyNOS2 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB18210 unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (C-Term) antibodyNOS2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (Thr177) antibodyNOS2 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) antibodyNOS2 适用: 人, 小鼠, 大鼠, 小鸡, 犬 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 533-696) antibodyNOS2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 2, Inducible (NOS2) (AA 533-696) antibodyNOS2 适用: 人 WB 宿主: 小鼠 Monoclonal C6 unconjugated
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应用备注
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ELISA: 1/20000-1/40000. Western blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunoflourescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
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别名
- NOS2
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背景
- Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including ncNOS (also designated NOS1) whose activity was first identified in neurons and maps at 12q24.2, and ecNOS (also designated NOS3), first identified in endothelial cells and mapping at 7q35-36. The inducible form of NOS, iNOS (also designated NOS2), is Ca2+-independent, expressed in a broad range of cell types and maps to 17cen -q12.Synonyms: Hepatocyte NOS, Inducible NO synthase, NOS type II, NOS-II, NOS2A, NOSII, iNOS
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分子量
- approx. 130.0 kDa
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基因ID
- 4843
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NCBI登录号
- NP_000616
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UniProt
- P35228
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途径
- Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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