EEF2 抗体
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Quick Overview for EEF2 抗体 (ABIN265392)
抗原
See all EEF2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of eEF2 protein. (region surrounding Ala50)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 32-233) antibody
EEF2 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 5B6 unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyKD Validated EEF2 适用: 人 WB, IF (p), ICC, FACS 宿主: 兔 Monoclonal 23GB1550 unconjugated Recombinant Antibody
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 1-858) antibodyEEF2 适用: 人, 小鼠, 大鼠, 斑马鱼, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) antibodyEEF2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 808-858) antibodyEEF2 适用: 人, 小鼠 IHC, IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 1-290) antibodyEEF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (N-Term) antibodyEEF2 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 2 (EEF2) (pThr56) antibodyEEF2 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Hamster WB, IHC, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))
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别名
- EEF2
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背景
- Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved.Synonyms: EF-2, EF2, Elongation factor 2
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分子量
- approx. 100 kDa
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基因ID
- 1938
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NCBI登录号
- NP_001952
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UniProt
- P13639
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途径
- AMPK Signaling
抗原
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