MAP3K8 抗体
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- 抗原 See all MAP3K8 抗体
- MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))
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适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MAP3K8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- 特异性
- This antibody detects endogenous levels of MAP3K8 / TPL-2 protein(region surrounding Pro284).
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity Chromatography using epitope-specific immunogen
- Top Product
- Discover our top product MAP3K8 Primary Antibody
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anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 121-220) antibody
MAP3K8 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 1-300) antibodyMAP3K8 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (pThr290) antibodyMAP3K8 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) antibodyMAP3K8 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 256-305) antibodyMAP3K8 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (Ser400) antibodyMAP3K8 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (AA 368-397), (C-Term) antibodyMAP3K8 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB03467 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (pSer290) antibodyMAP3K8 适用: 人, 大鼠, 小鼠, Cow, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (pSer400) antibodyMAP3K8 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) (pThr290) antibodyMAP3K8 适用: 人, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))
- 别名
- MAP3K8 / TPL-2 (MAP3K8 产品)
- 别名
- MAP3K8 antibody, COT antibody, EST antibody, ESTF antibody, MEKK8 antibody, TPL2 antibody, Tpl-2 antibody, c-COT antibody, Cot antibody, Cot/Tpl2 antibody, Est antibody, Estf antibody, Tpl2 antibody, D17TPL2 antibody, mitogen-activated protein kinase kinase kinase 8 antibody, MAP3K8 antibody, map3k8 antibody, Map3k8 antibody
- 背景
- The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.Synonyms: COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, ESTF, MAPK, Mitogen-activated protein kinase kinase kinase 8, Tumor progression locus 2, Tumor progression locus 2
- 分子量
- approx. 53 kDa
- 基因ID
- 1326
- NCBI登录号
- NP_005195
- UniProt
- P41279
- 途径
- PI3K-Akt Signaling, TCR Signaling
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