PRKAA1 抗体
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Quick Overview for PRKAA1 抗体 (ABIN265305)
抗原
See all PRKAA1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of AMPK1/AMPK2 protein (region surrounding Asp168).
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 1-300) antibody
PRKAA1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 28-257) antibodyPRKAA1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pThr172), (pThr183) antibodyPRKAA1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) antibodyPRKAA1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2B7 unconjugated
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 351-450) antibodyPRKAA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 351-450) antibodyPRKAA1 适用: 人, 小鼠, 大鼠, Pig, 绵羊 WB, IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) antibodyKD Validated PRKAA1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2685 unconjugated Recombinant Antibody
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-550) antibodyPRKAA1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D8 unconjugated
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 325-543), (Catalytic Subunit) antibodyPRKAA1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pSer487) antibodyPRKAA1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
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别名
- PRKAA1
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背景
- AMPK is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK alpha-1 chain, AMPK alpha-2 chain, PRKAA1, PRKAA2
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分子量
- approx. 63 kDa
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基因ID
- 5562
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NCBI登录号
- NP_006242
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UniProt
- Q13131
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途径
- AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
抗原
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