Cytokeratin 5 抗体
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北京 101111
Quick Overview for Cytokeratin 5 抗体 (ABIN264415)
抗原
See all Cytokeratin 5 (KRT5) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应 （详细）
- Also reacts to: KRT14
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产品特性
- Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5, Keratin type II cytoskeletal 5, 58 kDaCytokeratin
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纯化方法
- Purified
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免疫原
- Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratinenriched extract of Feline tongue epithelium and Mouse myeloma cells.
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亚型
- IgG2a
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anti-Keratin 5 (KRT5) (AA 316-590) antibody
KRT5 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 10C11E6 unconjugated
anti-Keratin 5 (KRT5) antibodyKRT5 适用: 人, 小鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Keratin 5 (KRT5) (AA 335-473) antibodyKRT5 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 5 (KRT5) (AA 1-273) antibodyKRT5 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4A7 unconjugated
anti-Keratin 5 (KRT5) (AA 316-590) antibodyKRT5 适用: 人 IHC 宿主: 小鼠 Monoclonal KRT5-3594 unconjugated
anti-Keratin 5 (KRT5) (AA 258-357) antibodyKRT5 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2C8E4 unconjugated
anti-Keratin 5 (KRT5) (AA 258-357) antibodyKRT5 适用: 人, 小鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3D6D4 unconjugated
anti-Keratin 5 (KRT5) antibodyKRT5 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 5 (KRT5) (Middle Region) antibodyKRT5 适用: 人 WB, IHC, IF, ICC, FACS 宿主: 小鼠 Monoclonal 5D3F7 unconjugated
anti-Keratin 5 (KRT5) (AA 331-430) antibodyKRT5 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Detects a 58 kDa band, corresponding to Keratin 5, and a 50 kDa band,corresponding to Keratin 14. Immunohistochemistry on Frozen Sections: 1-5 μg/mL. Does not work on Paraffin embedded tissue sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.4 mg/mL
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缓冲液
- PBS containing 0.08 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))
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别名
- Cytokeratin 5
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背景
- Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5
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基因ID
- 3852
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UniProt
- P13647
抗原
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