NAPA 抗体
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北京 101111
Quick Overview for NAPA 抗体 (ABIN264392)
抗原
See all NAPA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody specifically recognizes a-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize b-SNAP.
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产品特性
- Synonyms: NAPA, SNAP-alpha, SNAPA, Alpha-soluble NSF attachment protein,N-ethylmaleimide-sensitive factor attachment protein alpha
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纯化方法
- Standard chromatographic techniques.
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免疫原
- Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman a-SNAP protein and mouse myeloma cells.
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亚型
- IgG1
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应用备注
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Western Blot: 0.5-5 μg/mL. Immunoprecipitationon native and denatured samples: 2-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol without preservatives.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
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别名
- SNAP alpha
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背景
- SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or bSNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas a-SNAP is expressed in all mammalian tissues, b-SNAP is expressed only in brain.Synonyms: Alpha-soluble NSF attachment protein, N-ethylmaleimide-sensitive factor attachment protein alpha, NAPA, SNAP-alpha, SNAPA
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基因ID
- 8775
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UniProt
- P54920
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途径
- Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
抗原
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