Flow Cytometry. Monitoring of CD34-positive cells. Analysis of 8 % of myelomonocytes. Study of hematopoietic stem cell differentiation and proliferatiuon. Study of the c-kit gene product (SCF receptor). Study of a part of acute myeloblastic leukemia cells. Study of chronic myeloblastic leukemia cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
PBMC: Count the number of positive cells in peripheral blood. Add 10 µl of Mab to 50 µl suspended lymphocyte solution at 2 x 10e7. Mix gently and incubate for 15 minutes at 2-8°C.
限制
仅限研究用
缓冲液
PBS containing 0.08 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
CD117 reacts with 8 % of myelomonocytes and 75 % of CD34-positive cells. The hematopoietic stem cells are included in this fraction and therefore, this gene product is considered to have an important function for differentiation and proliferation of hematopoietic cells. CD117 also reacts with placenta, brain, a part of acute myeloblastic leukemia cells and chronic myeloblastic leukemia cells, but not with acute lymphoblastic leukemia cells. MW is 145 kDa.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR