KIT 抗体
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北京 101111
Quick Overview for KIT 抗体 (ABIN264306)
抗原
See all KIT 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- CD117 reacts against c-kit gene product (SCF receptor).
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产品特性
- Synonyms: SCFR, KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase
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纯化方法
- Purified
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免疫原
- Immunized with transfected cells with c-kit DNA in human leukemia cells (UT-7).
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亚型
- IgG1
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anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 350-440) antibody
KIT 适用: 人, 小鼠, 大鼠 FACS, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 321-520) antibodyKIT 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 343-527) antibodyKIT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (Internal Region) antibodyVerified KIT 适用: 人 WB, FACS, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr703) antibodyKIT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr721) antibodyKIT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 546-976) antibodyKIT 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) antibody (FITC)KIT 适用: 人, Cow, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 104D2 FITC
anti-Mast/stem Cell Growth Factor Receptor (KIT) antibodyKIT 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 1C5 unconjugated
anti-Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-285) antibodyKIT 适用: 人 WB, FACS, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry. Monitoring of CD34-positive cells. Analysis of 8 % of myelomonocytes. Study of hematopoietic stem cell differentiation and proliferatiuon. Study of the c-kit gene product (SCF receptor). Study of a part of acute myeloblastic leukemia cells. Study of chronic myeloblastic leukemia cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- PBMC: Count the number of positive cells in peripheral blood. Add 10 µl of Mab to 50 µl suspended lymphocyte solution at 2 x 10e7. Mix gently and incubate for 15 minutes at 2-8°C.
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限制
- 仅限研究用
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缓冲液
- PBS containing 0.08 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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别名
- CD117 / C-Kit
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背景
- CD117 reacts with 8 % of myelomonocytes and 75 % of CD34-positive cells. The hematopoietic stem cells are included in this fraction and therefore, this gene product is considered to have an important function for differentiation and proliferation of hematopoietic cells. CD117 also reacts with placenta, brain, a part of acute myeloblastic leukemia cells and chronic myeloblastic leukemia cells, but not with acute lymphoblastic leukemia cells. MW is 145 kDa.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR
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基因ID
- 3815
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UniProt
- P10721
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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