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CD28 抗体 (APC)

This anti-CD28 antibody is a 小鼠 单克隆 antibody detecting CD28 in FACS. Suitable for 人.
产品编号 ABIN264248
发货至: 中国

Quick Overview for CD28 抗体 (APC) (ABIN264248)

抗原

See all CD28 抗体
CD28

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD28 antibody is conjugated to APC

应用范围

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Flow Cytometry (FACS)

克隆位点

B-23
  • 特异性

    This antibody recats with CD28 receptor.

    产品特性

    Synonyms: TP44, T-cell-specific surface glycoprotein CD28

    亚型

    IgG1
  • 应用备注

    Flow Cytometry. Monitoring of activated T cells in peripheral blood. Study of T lymphocyte cytokine function. Study of B cell activation. Study of Cell-adhesion molecules relating T and B lymphocytes.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    实验流程

    PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

    限制

    仅限研究用
  • 缓冲液

    PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store the antibody undiluted at 2-8 °C. Do Not Freeze!
    Shelf life: One year from despatch.

    有效期

    12 months
  • 抗原

    CD28

    别名

    CD28

    背景

    Anti-human CD28 binds the 44 kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 Molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60 % -80 % T lymphocytes (95 % of CD4 and 50 % of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

    基因ID

    940

    UniProt

    P10747

    途径

    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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