CD5 抗体 (APC)
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北京 101111
Quick Overview for CD5 抗体 (APC) (ABIN264188)
抗原
See all CD5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Synonyms: Lymphocyte antigen T1/Leu-1, LEU1, T-cell surface glycoprotein CD5
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亚型
- IgG2a
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应用备注
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Flow Cytometry. This antibody is suitable for Monitoring of T cells subsets in peripheral blood, Analysis of Bcell subsets, Characterization of subtypes of T cell leukemias and lymphomas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- PBMC: Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of Antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturer's instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.
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限制
- 仅限研究用
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缓冲液
- PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch. -
有效期
- 12 months
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- CD5
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别名
- CD5
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背景
- Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85 % peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells.Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
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基因ID
- 921
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UniProt
- P06127
抗原
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