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Vimentin 抗体 (C-Term)

Name: Vimentin 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2613493
Availability: OutOfStock

This anti-Vimentin antibody is a 山羊多克隆 antibody detecting Vimentin in WB, IHC, ELISA, IF 和 FACS. Suitable for 人, 小鼠和大鼠.

(ABIN2613493) (5 μg/mL) staining of paraffin embedded Human Kidney. (Vimentin 抗体 (C-Term))

IHC

ABIN2613493 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). (Vimentin 抗体 (C-Term))

ABIN2613493 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (Vimentin 抗体 (C-Term))

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (Vimentin 抗体 (C-Term))

FACS

5 images

产品编号 ABIN2613493

发货至: 中国

Datasheet as PDF

Quick Overview for Vimentin 抗体 (C-Term) (ABIN2613493)

抗原

See all Vimentin (VIM) 抗体

Vimentin (VIM)

适用

人, 小鼠, 大鼠

宿主

山羊

克隆类型

多克隆

标记

This Vimentin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified

Discover our top product VIM Primary Antibody

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C-Term

原理

Vimentin

序列

QVINETSQHH DDLE

交叉反应

Cow, 犬, 人, 小鼠, Pig, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

亚型

IgG
anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

应用备注

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.

说明

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

Vimentin (VIM)

别名

VIM

背景

VIM, vimentin, FLJ36605

基因ID

7431, 22352, 81818

NCBI登录号

NP_003371

途径

Caspase Cascade in Apoptosis