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CD4 抗体 (Internal Region)

The 山羊 多克隆 anti-CD4 antibody is suitable to detect CD4 in samples from 人. It has been validated for FACS, WB, IHC 和 ELISA.
产品编号 ABIN2613402
发货至: 中国
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Our Local Distributor

中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD4 抗体 (Internal Region) (ABIN2613402)

抗原

See all CD4 抗体
CD4

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This CD4 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    CD4

    序列

    KNKEVSVKRV TQDPK

    特异性

    This antibody is expected to recognize isoform 1 (NP_000607.1) and isoform 2 (NP_001181943.1).

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 4 μg/mL.

    Western Blot: Approx 30 kDa band observed in Human Spleen and Bone Marrow lysates (calculated MW of 31.3 kDa according to NP_001181943.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    CD4

    别名

    CD4

    背景

    CD4, CD4 Molecule, CD4mut, CD4 antigen (p55), CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

    基因ID

    920

    NCBI登录号

    NP_000607, NP_001181943

    途径

    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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