AIF 抗体 (AA 183-195)
Quick Overview for AIF 抗体 (AA 183-195) (ABIN2613375)
抗原
See all AIF (AIFM1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 183-195
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原理
- AIFM1 (aa183-195)
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序列
- DDPNVTKTLR FKQ
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特异性
- This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).
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交叉反应
- Cow, 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-DDPNVTKTLRFKQ, from the internal region of the protein sequence
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5-7 μg/mL.
Western Blot: Approx 70 kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9 kDa according to NP_004199.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and NIH3T3 cells. Recommended concentration: 5-10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
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别名
- AIFM1
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背景
- AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
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基因ID
- 9131
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NCBI登录号
- NP_004199, NP_665811, NP_001124319
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途径
- Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect
抗原
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