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PCK1 抗体

There is 1 publication for this product available. The 兔 多克隆 anti-PCK1 antibody is suitable to detect PCK1 in samples from 小鼠, Zea mays, Saccharum, Pineapple, Oryza sativa, Megathyrsus maximus, Panicum virgatum, Miscanthus giganteus, Nannochloropsis oceanica, Setaria viridis, Spartina alterniflora, Spartina patens 和 Green bean. It has been validated for WB.
产品编号 ABIN249350
发货至: 中国
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Quick Overview for PCK1 抗体 (ABIN249350)

抗原

See all PCK1 抗体
PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

适用

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小鼠, Zea mays, Saccharum, Pineapple, Oryza sativa, Megathyrsus maximus, Panicum virgatum, Miscanthus giganteus, Nannochloropsis oceanica, Setaria viridis, Spartina alterniflora, Spartina patens, Green bean

宿主

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克隆类型

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多克隆

标记

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This PCK1 antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 原理

    Anti-PEPCK | PEP carboxykinase

    交叉反应 (详细)

    Ananas comosus, Miscantus giganteus, Mouse, Nannochloropsis oceanica, Oryza sativa, Panicum maximum, Panicum virgatum, Phaseolus vulgaris, Saccharum spp. hybrid clone C91-301,Setaria viridis, Spartina alterniflora, Spartina patens, Zea mays
    Not reactive in: Arabidopsis thaliana, Pinus yunnanensis

    预测反应

    Brassica napus,Chromera velia,Cucumis sativus,Flaveria sp.,Lycopersicon esculentum,Medicago sativa,Oryza sativa,Urochloa panicoides,Zoysia japonica,Zea mays

    纯化方法

    Serum

    免疫原

    KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0
  • 应用备注

    1 : 1000 (WB)

    说明

    due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 200 μL of sterile water

    缓冲液

    Serum

    注意事项

    Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

    储存条件

    -20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

  • 抗原

    PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

    别名

    PEPCK

    背景

    Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.

    分子量

    73 | 78 kDa

    基因ID

    541622

    UniProt

    Q9SLZ0

    途径

    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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