FKBP4 抗体 (HRP)
Quick Overview for FKBP4 抗体 (HRP) (ABIN2486531)
抗原
See all FKBP4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Detects ~52 kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
-
交叉反应
- 犬, Hamster, 人, 小鼠, 大鼠
-
纯化方法
- Protein G Purified
-
免疫原
- Synthetic peptide corresponding to the residues of human FKBP52
-
亚型
- IgG2a
-
-
anti-FK506 Binding Protein 4, 59kDa (FKBP4) (AA 301-410) antibody
FKBP4 适用: 人 WB, IP, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 5C11 unconjugated
anti-FK506 Binding Protein 4, 59kDa (FKBP4) (AA 2-459) antibodyFKBP4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-FK506 Binding Protein 4, 59kDa (FKBP4) (AA 1-459) antibodyFKBP4 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-FK506 Binding Protein 4, 59kDa (FKBP4) (AA 1-459) antibodyFKBP4 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-FK506 Binding Protein 4, 59kDa (FKBP4) (AA 220-459) antibodyFKBP4 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-FK506 Binding Protein 4, 59kDa (FKBP4) antibodyFKBP4 适用: 人 WB, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal Hi52C unconjugated
anti-FK506 Binding Protein 4, 59kDa (FKBP4) antibodyFKBP4 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-FK506 Binding Protein 4, 59kDa (FKBP4) antibody (PE)FKBP4 适用: 人 WB, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal Hi52C PE
anti-FK506 Binding Protein 4, 59kDa (FKBP4) antibody (PerCP)FKBP4 适用: 人 WB, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal Hi52C PerCP
anti-FK506 Binding Protein 4, 59kDa (FKBP4) antibody (APC)FKBP4 适用: 人 WB, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal Hi52C APC
-
-
应用备注
-
- WB (1:2000)
- IHC (1:250)
- ICC/IF (1:1000)
- IP (5 μg)
- optimal dilutions for assays should be determined by the user.
-
说明
-
0.5 μg/ml was sufficient for detection of FKBP52 in 20 μg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Conjugated antibodies should be stored at 4°C
-
-
- FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))
-
别名
- FKBP52
-
背景
- HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
-
基因ID
- 2288
-
NCBI登录号
- NP_002005
-
UniProt
- Q02790
-
途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
抗原
-
