SOD1 抗体 (N-Term) (Atto 390)
Quick Overview for SOD1 抗体 (N-Term) (Atto 390) (ABIN2486233)
抗原
See all SOD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- Recognizes a conformation specific epitope where the beta barrel is unfolded.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Protein A Purified
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免疫原
- Within 50 AA of N-terminal region of SOD1, unfolded beta barrel region
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anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 1-154) antibody
SOD1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 1, Soluble (SOD1) antibodyKO Validated SOD1 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 1, Soluble (SOD1) (C-Term) antibodyKO Validated SOD1 适用: 人 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibodySOD1 适用: 人, 马 WB, IHC, IP, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 6-100) antibodySOD1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), FACS, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibodySOD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibodySOD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody (APC)SOD1 适用: 兔 WB, IHC 宿主: 豚鼠 Polyclonal APC
anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibodySOD1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibodySOD1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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1μg/ml of ABIN2486233 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- SOD1 (Superoxide Dismutase 1, Soluble (SOD1))
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别名
- SOD1
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背景
- Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (UbetaB), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with Anti-SOD1 (EDI) (SPC-206D) which detects an exposed dimer interface (EDI) of SOD1.
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基因ID
- 6647
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UniProt
- P00441
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途径
- Sensory Perception of Sound, Transition Metal Ion Homeostasis
抗原
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