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DNMT1 抗体 (AA 637-650) (HRP)

DNMT1 适用: 人 WB, IHC, IP, ChIP 宿主: 小鼠 Monoclonal 60B1220-1 HRP
产品编号 ABIN2486007
发货至: 中国
  • 抗原 See all DNMT1 抗体
    DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
    抗原表位
    • 26
    • 22
    • 16
    • 14
    • 12
    • 12
    • 10
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 637-650
    适用
    • 203
    • 56
    • 46
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 156
    • 47
    • 1
    • 1
    小鼠
    克隆类型
    • 144
    • 61
    单克隆
    标记
    • 82
    • 17
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    • 10
    • 6
    • 6
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    • 6
    • 3
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    • 2
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    This DNMT1 antibody is conjugated to HRP
    应用范围
    • 133
    • 68
    • 45
    • 41
    • 33
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    • 28
    • 18
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
    特异性
    Detects ~180 kDa. It will cross-react with mouse DNMT1.
    交叉反应
    鱼, 人, 小鼠, 斑马鱼
    纯化方法
    Protein G Purified
    免疫原
    Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
    克隆位点
    60B1220-1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product DNMT1 Primary Antibody
  • 应用备注
    • WB (1:1000)
    • IHC (1:1000)
    • optimal dilutions for assays should be determined by the user.
    说明

    2 μg/ml of ABIN2486007 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
    别名
    DNMT1 (DNMT1 产品)
    别名
    cb1075 antibody, cb983 antibody, dmnt1 antibody, fb05h01 antibody, mta antibody, wu:fb05h01 antibody, ADCADN antibody, AIM antibody, CXXC9 antibody, DNMT antibody, HSN1E antibody, MCMT antibody, DDM2 antibody, DECREASED DNA METHYLATION 2 antibody, DMT01 antibody, DMT1 antibody, DNA METHYLTRANSFERASE antibody, DNA METHYLTRANSFERASE 01 antibody, DNA METHYLTRANSFERASE 1 antibody, MET2 antibody, METHYLTRANSFERASE 2 antibody, METHYLTRANSFERASE I antibody, METI antibody, methyltransferase 1 antibody, Cxxc9 antibody, Dnmt antibody, Dnmt1o antibody, MTase antibody, Met-1 antibody, Met1 antibody, MommeD2 antibody, m.MmuI antibody, DNA methyltransferase 1 antibody, DNA (cytosine-5-)-methyltransferase 1 antibody, methyltransferase 1 antibody, DNA methyltransferase (cytosine-5) 1 antibody, DNMT1 antibody, dnmt1 antibody, Dnmt1 antibody, MET1 antibody
    背景
    Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
    基因ID
    1786
    NCBI登录号
    NP_001370
    UniProt
    P26358
    途径
    SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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